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中文摘要：
扩增了从甘蓝柱头蛋白质谱鉴定中获得的泛素蛋白BoSU03基因的编码序列。序列分析表明BoSU03与甘蓝泛素蛋白Bo1003815的核苷酸序列相似性最高,达98%,含有臂重复结构域,属于植物泛素蛋白家族。为探究BoSU03是否为自交不亲和S位点受体激酶（SRK）下游的互作蛋白,以SRK与ARC1相互作用为对照,利用GAL酵母双杂交系统对SRK与BoSU03进行了相互作用验证,结果表明,BoSRK/BoSU03的转化酵母在四缺平板SD/-Ade/-His/-Leu/-Trp/x-α-gal/25 mmol 3-AT上长势较好,显色较深,进一步检测发现BoSRK/BoSU03转化酵母的β-半乳糖苷酶活性值大于BoSRK/BoARCl转化酵母的活性值,这为进一步分析鉴定BoSU03是否参与SRK下游信号传导过程提供了依据。
英文摘要：
In this study,the coding sequences of ubiquitin protein BoSU03 identified by Brassica oleracea stigma protein mass spectrometry were amplified.Nucleotide sequence analysis showed that it was highly identical with nucleotide sequence of ubiquitin protein Bol003815 in Brasscia oleracea,BoSU03 contained ARM repeat domain and belonged to Plant U-box protein family.In order to analyze the interaction between BoSU03 and SRK,GAL4 two-hybrid system was used here,and the interaction between SRK and ARC1 was used as control.The results showed that the yeast cells transformed with BoSRK/BoSU03 could grow better and turn deeper blue on SD/-Ade/-His/-Leu/-Trp/x-α-gal/25 mmol 3-AT nutritional media.And the level of β-Galactosidase activity of BoSRK/BoSU03 was higher than that of BoSRK/BoARC1.These results will provide the basis for further analysis whether BoSU03 participates in SRK downstream SI signaling pathway.
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