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中文摘要：
目的：研究黄芪种子内源抑制物质活性及其存在的部位。方法：采用浸提法对黄芪种子不同部位进行浸提,获得不同溶剂、不同浓度、不同浸提次数的浸提液,以白菜、小麦种子进行生物活性测定。结果：黄芪种子浸提液可显著抑制小麦和白菜种子的萌发及幼苗生长,且抑制物的活性随质量浓度的增大而增强,当质量浓度达到0.20 g/m L时,小麦和白菜种子萌发率均为0。黄芪种子不同溶剂浸提液抑制活性存在较大差异,其中水溶性浸提液大于醇溶性浸提液,且随着浸提次数的增加,浸提液的抑制活性呈现逐渐减弱的趋势。种皮与种仁都含有活性极强的抑制物质,后者强于前者。结论：黄芪种子中存在活性较强的内源抑制物,且多为水溶性成分,其种仁中含量高于种皮。
英文摘要：
Objective：To investigate the activity and the location of the endogenesis inhibitory substances in seed of Astragalus membranaceus. Method： For obtaining extracts of different solvent, concentrations and extracting times, extraction method was adopted in different tissues of Astragalus membranaceus seeds. Biological activities were determined by wheat and Brassica sp seed. Result：The extract dramatically inhibited the germination and the growth of wheat and Brassica sp seed and the inhibitory activity was positively related to the concentration of the extracts. When the concentration reached 0.20 g/mL, wheat and Brassica sp seed did not germinate. Distinct difference in inhibitory activity in extracts of different solvent from Astragalus membranaceus seeds were found, of which the water-soluble extracts＇ inhibitory activity was more significant than alcohol-soluble extracts. With the increased extracting times, the inhibitory activity showed a trend of weakening. The seed coat and kernel both had inhibitory substances, and the latter was stronger than the former. Conclusion： Highly active inhibitory substances were found in the seed of Astragalus membranaceus, and most of them were water-soluble ingredients. The active inhibitory substances in seed kernel was higher than seed coat in Astragalus membranaceus seeds.
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