中文摘要：

目的：运用紫外分光光度法测定黑面神药材中总三萜类成分的含量，从而为有效控制药材质量提供依据。方法：以药材中主要成分三萜类的熊果酸为考察指标，以5％香草醛一冰乙酸及高氯酸为显色剂，采用紫外分光光度法在547nm波长处测定样品的吸光度。结果：总三萜类成分在8．28～49．68μg范围内呈良好线性关系，其回归方程为Y=10．507X＋0．0077，r=0．9995，黑面神中总三萜类成分的含量约为3．89％。结论：紫外分光光度法适合黑面神药材中总三萜类成分的含量测定，该方法操作简便，结果稳定，重现性好。

英文摘要：

Objective ： To determine the total tritepenoidic in Breynia fruticosa. Methods ： Total tritepenoidic was determined by spectrometry using ursolic acid as standard and 5 % vanillin-glacial acetic and perchlorie acid as color-developing agents. Results ： The calibration curve was linear（r=0. 9995）in the range of 8.28-49.68μg,and the curve was Y=10. 507X＋0. 0077,the content was about 3.89 %. Conclusion： The spectrophotometry is suitable for the determination of total tritepenoidic from Breynia fruticosa. This method operated eonvenientely,the result was stable,and has good repeatability.