中文摘要：

目的：探讨血清胸苷激酶（S-TK1）检测在甲状腺良恶性疾病鉴别诊断中的应用价值。方法：收集84例甲状腺疾病患者术前血清标本,采用CIS-1型印迹免疫—增强发光系统对S-TK1的浓度进行检测,以S-TK1浓度〉2.00pmol/L定义为阳性检出。对比分析S-TK1的阳性检出率及浓度在术后病理证实的甲状腺疾病良恶性组间差异。应用受试者工作特征（ROC）曲线分析,获得鉴别甲状腺良恶性疾病的最佳S-TK1浓度阈值。结果：甲状腺疾病良性组S-TK1阳性检出率为28.07%（16/57）明显低于恶性组阳性检出率74.07%（20/27）（P=0.000）;恶性组S-TK1浓度[（4.90±3.51）pmol/L]明显高于良性组浓度[（1.52±1.12）pmol/L],差异有统计学意义（P=0.000）;以S-TK1=2.00pmol/L作为诊断界值时,诊断灵敏度与特异度分别为74.1%和71.9%,而以S-TK1=3.22pmol/L作为诊断界值时,灵敏度与特异度分别为70.4%和89.5%,诊断效能明显高于前者（Z=4.873,P〈0.000 1）。结论：S-TK1可为甲状腺良恶性疾病的鉴别诊断提供一定的参考价值。

英文摘要：

Objective：To investigate the application value for serum cytoplasmic thymidine kinase 1 （S-TK1） in the differential diagnosis of thyroid benign and malignant disease. Methods：Serum from 84 patients with thyroid disease was collected prior to surgery. The method of CIS-1 imprinting immune-enhanced was used to detect the level of S-TK1 in thyroid disease. The S-TK1 concentration of 2.00 pmol/L was defined as a positive detection. Analyzed the S-TK1 positive rate and concentration in the benign and malignant group of thyroid disease. ROC curve was used to obtain the optimal threshold of the S-TK1 concentration. Re- suits： The positive rate of S-TK1 in benign group （8.070%, 16/57） were significantly lower than that in ma- lignant group（74.07% ,20/27） （ P=0. 000）. S-TK1 concentration of the thyroid malignant group [（4.90±3.51）pmol/L] was significantly higher than that of the benign group[-（1.52±1.12）pmol/L] （P ： 0. 000）. ROC curve revealed that when 2.0 pmol/L was defined as the best threshold, the sensitivity and specificity were 74.1~ and 71.9M, respectively. And when the sensitivity and specificity were 70.4% and 89.5%, respectively, the best threshold was 3.22 pmol/L. The diagnostic performance of the latter was superior to the former （ Z=4. 873, P〈0. 000 1）. Conclusion： S-TK1 may provide certain value in the differential diagnosis of thyroid benign and malignant disease.