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Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum

ISSN号：1674-7968
期刊名称：《农业生物技术学报》
时间：0
分类：S512.1[农业科学—作物学]
作者机构：[1]Triticeae Research Institute, Sichuan Agricultural University, Ya＇an 625014, P.R.China, [2]Key Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education/Sichuan Agricultural University,Ya＇an 625014, P,R. China
相关基金：Acknowledgements This work was supported by the National High Technology Research and Development Program of China （863 Program, 2006AA10Z179, 2006AA10ZEF87）, National Natural Science Foundation of China （30300219 and 30571163）, and the Foundation for the Authors of National Excellent Doctoral Dissertation of China （200357 and 200458）. Wei Yuming was supported by the Program for New Century Excellent Talents in Universifies of China. Zheng Youliang was supported by the Program for Changjiang Scholars and Innovative Research Teams in Universities of China （IRT0453）.

作者： WANG Han-yan[1], WEI Yu-ming[1,2], ZE Hong-yan[1,2], ZHENG You-liang[1,2]

关键词：硬粒小麦, α-醇溶蛋白, 基因克隆, 序列分析, durum wheat, α-gliadin, gene clone, sequence analysis

中文摘要：

麦胶蛋白质基因的三个编码序列，作为 Gli2_Du1， Gli2_Du2 和 Gli2_Du3 设计了，从 Triticum 硬质小麦就职 CItr5083 的 genomic 脱氧核糖核酸被孤立。Gli2_Du1 和 Gli2_Du2 包含 945 和 864 bp，分别地编码有 314 和 287 氨基酸残余的成熟蛋白质。Gli2_Du3 在在编码区域发生上由于站鳕鱼作为假基因被认出。假基因，通常发生在麦胶蛋白质家庭，被归因于单个基础变化 C → T。氨基酸顺序从这些推断基因序列与α - 麦胶蛋白质蛋白质的典型结构被描绘，包括有毒的序列(PSQQQP ) 。肽部分 PF (Y) PP (Q) 被认为是重复领域的一个额外的单位，稍微从以前的报告分叉。六半胱氨酸残余在二个唯一的领域以内被观察。种系发生的分析显示出 Gli2_Du2， Gli2_Du3 是仔细与染色体 6A 上的基因有关，而 Gli2_Du1 似乎与染色体 6B 上的基因更相应。

英文摘要：

Three coding sequences of gliadins genes, designed as Gli2_Dul, Gli2_Du2 and Gli2_Du3, were isolated from the genomic DNA of Triticum durum accessions CItr5083. Gli2_Dul and Gli2_Du2 contain 945 and 864 bp, encoding the mature proteins with 314 and 287 amino acid residues, respectively. Gli2_Du3 is recognized as a pseudogene due to the stop codon occurring in the coding region. The pseudogenes, commonly occurring in gliadins family, are attributed to the single base change C→T. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-gliadin proteins, including the toxic sequences （PSQQQP）. The peptide fraction PF（Y）PP（Q）is thought to be an extra unit of repetitive domain, slightly diverging from the previous report. Six cysteine residues were observed within two unique domains. Phylogenetic analysis showed Gli2_Du2 and Gli2_Du3 were closely related to the genes on chromosome 6A, whereas Gli2_Dul seems to be more homologous with the genes on chromosome 6B.

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