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中文摘要：
为在干旱忍耐的帮助标记的选择使用的分子的标记的选择提供有用信息，发现更多的候选人基因并且在干旱应力下面在基因表示规定完成信息差距是必要的。在这研究，我们孤立四差别从由抑制的一根干旱容忍的生来的线的叶子的表示 cDNA 碎片减少性的杂交和反向的北杂交，并且由量的即时 PCR 响应干旱应力与不同干旱公差在六根生来的线之中验证了他们的微分表示模式。顺序类似分析显示了表示 cDNA 显示出的四差别中的那二个相同到编码像胰岛素的丝氨酸朊酶的基因 DegP，和基因 PGAM -- 分别地编码余因子无关的 phosphoglyceromutase 的 i。相应于四 cDNA 碎片的基因的表情在大多数六根生来的线在干旱压力处理以后在 6 h 被减少，然后在三根容忍的生来的线与进一步的应力回到了控制水平。基因 PGAM 的表示 -- 我和相应于碎片 E4 和 F4 的基因在容忍的生来的线被增加到高水平 81565。在二根干旱敏感的生来的线(Dan340 和 ES40 ) ，这些基因的表示仍然是下面调整的。响应干旱应力的这些基因的可能的机制被讨论。这些结果显示干旱容忍的生来的线起来调整干旱容忍的候选人基因的表示，相反干旱敏感的生来的线下面调整基因的表示。
英文摘要：
To provide the useful information for the choice of molecular marker used in marker-assisted selection of drought tolerance, it is necessary to find out more candidate genes and fulfill the information gaps in gene expression regulation under drought stress. In this study, we isolated four differentially expressed cDNA fragments from leaves of a droughttolerant inbred line by suppression subtractive hybridization and reverse Northern hybridization, and validated their differential expression patterns among six inbred lines with different drought tolerance in response to drought stress by quantitative real-time PCR. Sequence similarity analysis indicated that two of four differentially expressed cDNA showed homology to gene DegP encoding trypsin-like serine protease, and gene PGAM-i encoding cofactor-independent phosphoglyceromutase, respectively. Expressions of the genes corresponding to four cDNA fragments was decreased at 6 h after drought stress treatment in most of the six inbred lines, and then returned to the control level with further stress in three of the tolerant inbred lines. The expression of the gene PGAM-i and the genes corresponding to fragments E4 and F4 were increased to a high level in tolerant inbred line 81565. In the two drought-sensitive inbred lines （Dan340 and ES40）, the expression of these genes was still down-regulated. The probable mechanisms of these genes in response to drought stress were discussed. These results indicated that the drought-tolerant inbred lines upregulated the expression of the drought-tolerant candidate genes, in contrast, drought-sensitive inbred lines downregulated the expression of the genes.
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