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中文摘要：
为寻找化学诱导表达基因，利用mRNA差异显示方法，从除草剂安全剂AD-67（苯叉酰胺）诱导处理的水稻品种9311的幼苗中筛选分离得到1个诱导表达增强的cDNA片段。该片段在处理后6h表达明显增强，直到第5天仍然有表达。从poly—gel上回收片段并测序，核酸同源序列分析显示它与水稻多个EST和cDNA序列一致性达到100％，氨基酸序列分析表明它与水稻、四膜虫、隐球酵母等真核生物的Yippee基因相似性达60％～84％。序列经延伸得到831bp的cDNA，由此设计引物并进行RT—PCR分析，结果说明该基因在除草剂安全剂AD-67诱导后表达增强。
英文摘要：
A safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript increased 6 h after the rice seedlings were sprayed with the safener solution and 5 days later, the expression could still be detected. The fragment was recycled from the poly-gel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice data base, and showed 60%- 84 % homologous to the gene Yippee in several eukaryotes in amino acid sequence. The fragment was extended to a 831 bp cDNA, a primer was designed and RT-PCR analysis supported the induction result.
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