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中文摘要：
以单倍体诱导系stock6诱导的单倍体为材料，用6个浓度秋水仙素人工加倍玉米单倍体，比较不同秋水仙素浓度处理不同部位的加倍效果。单倍体雌雄育性的自然恢复结果表明：不同基凶型单倍体存在很大差异，部分单倍体植株无雌穗，有雌穗的单倍体自然育性恢复为25％～50％，单穗结实1～84粒；雄穗自然散粉率最高仅为14．3％。秋水仙素加倍试验表明：浸种法和浸芽法对单倍体药害程度较轻，但散粉率和加倍率均低；浸根法处理的单倍体散粉率和加倍率均高于对照，但受低成活率影响，100％加倍率的单倍体散粉率仅有6．7％；注射法的处理效果最好，获得的加倍植株最多。
英文摘要：
The doubling methods of maize haploid from different genotype female were studied preliminarily with the materials induced by Stock6. The natural fertility of ear and inflorescence of the induced haploid which was different between genotypes was observed. Few of haploid could not have ears. Tassels of haploid plants were found to be almost completley sterile. The highest shedding plant rate was 14.3%. Fertility of ears was studied by pollinating them with the pollen from diploid inbred lines, the cross resulting in 25% to 50% haploid ears carrying kernels. And 1 to 84 kernels per ear of haploid plant were obtained o The haploids were treated separately with different colchicines concentration and 2% DMSO, of four haploid colchicines doubling treatments （immersing seeds, immersing buds, immersing seedling roots of 2 - 3 leaf stage, microinjecting in six leaf stage）, the drug injured plants became more and more with the rising of the colchicines concentration. Plant survival rates of immersing seeds and immersing buds were light but lower shedding plant rate and doubling frequencies. The doubling frequencies of immersing seedling roots of 2 - 3 leaf stage at 0.4% - 0.6% concentration performed better, but the doubling frequencies of it are 100% but shedding plant rate were 6.7% at 0.6% concentration with low survival rate. These results showed that microinjecting in six leaf stage would be better options for chromosome doubling.
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