中文摘要：

目的 探讨大肠癌中Hedgehog信号通路相关基因Sonic hedgehog（SHH）的表达及其临床意义.方法 应用RT-PCR及免疫组织化学法（Envison二步法）检测本院2008年12月至2009年4月收集的43例大肠癌组织和距离癌10 cm以上的癌旁组织的SHHmRNA及蛋白的表达情况,并与20例非肠癌患者的正常大肠组织对照,分析其与大肠癌患者临床和病理各变量的相关性.结果 凝胶成像分析显示阳性表达的样本SHH mRNA片段大小为280 bp,与理论值相符,未表达的样本则未见相应条带.大肠癌组织SHHmRNA表达阳性率为27.9%（12/43）,与癌旁组织的18.6%（8/43）差异无统计学意义（P＞0.05）,但均显著高于正常大肠黏膜的0%（0/20）（均P＜0.05）.SHH mRNA在大肠癌组织的表达强度显著高于癌旁组织（P＜0.05）,在正常大肠黏膜则未见其表达.免疫组织化学显示SHH蛋白在大肠癌组织和癌旁组织均有阳性表达,细胞膜及胞质内出现棕黄褐色颗粒,背景不着色;正常大肠黏膜阴性表达.SHH蛋白在大肠癌组织的表达阳性率显著高于正常大肠黏膜[25.6%（11/43）比0%（0/20）,P＜0.05],在大肠癌组织与癌旁组织、癌旁组织与正常大肠黏膜的表达阳性率差异则无统计学意义（均P＞0.05）.SHH蛋白的表达强度大肠癌组织＞癌旁组织＞正常大肠黏膜（均P＜0.05）.大肠癌组织中的SHH mRNA和蛋白表达强度与患者的年龄、性别、临床分期、肿瘤部位、肿瘤浸润深度、病理分型等变量均无明显关系（均P＞0.05）.结论 SHH mRNA和蛋白在大肠癌组织中表达显著增强,但与临床和病理各变量无关联.

英文摘要：

Objective To evaluate the expression of Hedgehog signaling pathway related Sonic hedgehog （SHH） gene in colorectal carcinoma （CRC） and to explore its clinical significance. Methods Between December 2008 and April 2009, 43 specimens of fresh CRC tissues and adjacent tissues （〉10 cm away from the cancer margin） were collected and detected for their expressions of SHH mRNA and proteins with RT-PCR and immunohistochemistry （Envision method）, and compared with 20 specimens of normal colorectal tissues from non- CRC patients as controls. The associations of SHH mRNA and proteins expressions and clinicopathological variables were analyzed. Results Gel-imaging analysis displayed a 280 bp band in SHH mRNA-positive specimens in consistency with theoretical value, which was not seen in SHH mRNA-negative specimens. The rate of SHH mRNA expression in CRC tissues （27.9%, n=12） was not statistically different from that in the adjacent tissues （18.6%, n=8） （P〉0.05） , but the both were significantly higher than that in the normal mucosa （0%, n=0） （all P〈0.05）. The expression intensity of SHH mRNA was significantly higher in CRC tissues than that in the adjacent tissues （P〈0.05） but not detected in normal colorectal tissues （P〈0.05）. Immunohistochemistry demonstrated positive expression of SHH proteins in both CRC tissues and adjacent tissues, appearing as yellow-brown particles with unstained background in the cell membrane and cytoplasm. The expression of SHH protein was negative in the cell membrane of normal colorectal mucosa. The rate of SHH protein expression in CRC tissues was remarkably higher than that in normal colorectal mucosa [25.6% （11/43） vs 0% （0/20） , P〈0.05]. There was no statistical difference in the positive rate of SHH protein expression between CRC and adjacent tissues, and between adjacent tissues and normal colorectal mucosa （all P〉0.05）. The CRC tissues showed the highest intensity of SHH protein expression, followed by adjacent tissues