测定了四川7个地方山羊品种(类群)43个个体的mtDNA控制区全序列,结果表明:山羊mtDNA控制区全序列长度为1212bp或1213bp,A+T含量(59.9%)明显高于G+c含量(40.1%)。共检测到74个变异位点,序列均为中性突变,核苷酸多样度为1.686%,这些差异共定义了27种单倍型,单倍型多样度为0.966,遗传多样性较为丰富,品种(类群)间存在不同程度的遗传分化。7个地方山羊品种(类群)间的遗传距离变异范围为0.0017~0.0306。用MEGA软件的NJ法构建单倍型序列的系统发育无根树,结果表明四川地方山羊品种(类群)有两个母系来源,但是否就对应于角笋骨羊(Capra aegagrus)和捻角山羊(Capra falconeri)两个野生祖先,还有待于进一步研究。
Complete sequence of mitochondrial DNA(mtDNA) control region was sequenced in 43 individuals from 7 indigenous goat (Capra hircus) breeds (groups) in Sichuan province. The complete sequence of mtDNA control region was 1 212 or 1 213 base pair, and the content of A+T (59.9 % )was higher than that of G+C(40.1% )significantly. 74 sites were polymorphic and all of these sequences were neutral mutation. All sequence polymorphic sites defined 27 haplotypes. High nucleotide diversity (1. 868 %) and haplotype diversity (0. 966) were manifested. These results suggested richer genetic diversity within Sichuan indigenous goat populations and genetic divergence among them. The Kimura 2-parameter distance among breeds (groups)varied from 0. 001 7 to 0. 030 6. Phylogenetic unrooted trees constructed with neighbour-joining methods showed two maternal origins in Sichuan indigenous goats. More detailed studies should be continued to indicate whether the two maternal origins are Capra aegagrus and Capra falconeri.