中文摘要：

目的：探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白（TPEF）、信号转导子和转录激活子1（STATI）在大肠癌中的表达水平及其意义。方法：采用SYBR GreenI荧光定量RT—PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRNA的表达进行检测，并分析其表达情况与临床病理资料的相关性。结果：癌组织中TPEFmRNA及STAT1 mRNA的相对表达水平明显低于相应正常组织（P〈0．05）；肿瘤TNM分期中T3／T4癌组织表达水平明显低于TI／T2癌组织（P〈0．05）；合并淋巴结转移的组织中表达水平亦低于无淋巴结转移者（P〈0．05）；TPEFmRNA与STAT1mRNA的表达水平呈正相关（r癌组织=0．537，r正常组织=0．851，P〈0．001）。结论：TPEF、STAT1基因在大肠癌组织中低表达，与大肠癌的转移相关，可作为大肠癌恶性潜能的检测指标之一。

英文摘要：

Objective：To investigate the expression levels and significance of transmembrane protein endothelial factor（ TPEF ） and signal transducer and activaon of transcription 1 （STAT1） in colorectal cancer. Methods： Expression levels of TPEF mRNA and STATI mRNA were detected by SYBR Green I quantitative real-time PCR in colorectal cancer and corresponding normal mueosa in 50 patients; analysis was performed to study on the correlation between the expression levels of TPEF and STATI and clinicopathological parameters,and on the relationship between expression level of TPEF with STAT1. Results： Expression levels of TPEF mRNA and STAT1 mRNA in colocrectal cancer were significantly decreased than that in normal tissue（P 〈0.05） ; and more frequently in stage T1/T2 cancer group than in stage T3/T4 group（P 〈0.05） ; in non-lymph node migrate group were significantly higher than that of the lymph node migrate group（P 〈 0.05） ; and there was a positive correlation between the expression level of TPEF mRNA and STAT1 mRNA （r =0. 537,r 1 =0. 851 ,P 〈0. 001 ）. Conclusion： TPEF and STAT1 were lowerly expressed in eolorectal cancerous tissues,and highly correlated with invasion and metastasis,may serve as a biomarker for invasiveness and metastasis of colorectal cancer.