中文摘要：

本研究以90只南江黄羊为材料，对山羊GH基因（gGH）部分片段进行了PCR-RFLP分析，并对GH基因的PCR-RFI，P与早期生长发育性状的相关性进行了研究。结果表明扩增产物片段长度为985bp，包括gGH内含子3、4以及外显子3、4、5的全部序列。分别用限制性内切酶FokⅠ和AciⅠ酶切分析扩增产物，均具有多态性，其中内切酶FokⅠ酶切产生GG、GA和AA3种基因型，频率分别为0．522、0．289和0．189，等位基因G的频率（0．666）高于等位基因A（0．334）；AciⅠ酶切产生CC、TC和TT3种基因型，频率分别为0．467、0．322和0．211，等位基因C的频率（0.628）高于等位基因T（0.372）。x^2检验结果表明2种限制性内切酶产生的基因型频率在群体中都处于Hardy-Weinberg非平衡状态。进一步对gGH的PCR-RFLP与早期生长发育的相关分析表明，FokⅠ酶切位点具有GA基因型的个体和AciⅠ酶切位点具有TC基因型个体的各年龄阶段体重、体高和体长的最小二乘均值均显著或极显著高于其它基因型的个体（0．05〈P〈0．01或P〈0．01）。

英文摘要：

The partial fragments of GH gene polymorphisms of 90 Nanjiang Brown goats were detected by PCR-RFLP. Association of PCR- RFLP at the GH gene with early growth and developmental traits in the Nanjiang Brown goats was also analyzed. The result showed that the sequence of PCR-amplified products was 985 bp which included goat GH gene sequences of exon 3, exon 4, exon 5 and intron 3, intron 4. The PCR products were digested by two kinds of restriction endonuclease named Fok Ⅰ and Aci Ⅰ , respectively. Both of them had polymorphisms . The FokⅠ produced GG, GA and AA genotypes which frequencies were 0. 522, 0. 289 and 0. 189, but the allele frequency of G （0. 666） was higher than that of A （0. 334） . The Aci Ⅰ produced CC, TC and TT genotypes which frequencies were 0. 467,0. 322 and 0. 211, respectively, but the allele frequency of C （0. 628） was higher than that of T （0. 372）. Chi-square test revealed that the genotype frequencies produced by restriction endonucleases were non-equilibrium Hardy-Weinberg.Association analysis of PCR-RFLP at the GH gene with early growth and developmental traits indicated that the least square means of weight, body height and body length with GA and TC genotypes were both significantly higher than that with other genotypes at different month ages （0. 05〈P〈0.01 or P〈0. 01）.