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中文摘要：
在这研究，二 enolase 基因从中国橡树蚕被孤立并且描绘， Antheraea perny，它被指定为 enolase 我和 II 分别地。enolase 我 cDNA 顺序是有编码 433 氨基酸的 1302 bp 的一个开的读物框架(ORF ) 的 1712 bp。enolase II cDNA 顺序是有编码 431 氨基酸的 1296 bp 的 ORF 的 1549 bp。二基因的氨基酸序列分享 enolase 的几个保存特征 / 地点。我显示出的 Antheraea pernyi enolase 93%97% 顺序身份到迄今为止可得到的 lepidopterans 的 enolases，到另外的无脊椎动物的 enolases 的 75%82% 身份，到包括脊椎动物的另外的有机体的 enolases 的 60%72% 身份，植物，和真菌。Antheraea pernyi enolase II 显示出 84% 身份到 Bombyx mori enolase II，而是 60% 身份到 A。pernyi enolase 我。在种系发生的树上， enolase II 从 A 定序。pernyi 和 B。mori 清楚地包括 A 与更高的有机体的 enolase 序列的多数被分开。pernyi 和 B。我定序的 mori enolase。由顺序比较和种系发生的分析，我们从 A 建议那 enolase II。pernyi 和 B。mori 可以是 enolase 总科的一个新成员。Antheraea pernyi enolase 我而 enolase II mRNA 在睾丸和卵巢明确地被表示， mRNA 在所有测试纸巾被发现，建议 enolase II 基因的产品可能与复制有关。抄本许多 A。pernyi enolase 我基因在冷吃惊以后并且显著地是显著地下面调整的在热吃惊以后起来调整，建议那 A。pernyi enolase 我基因可能由温度应力是可诱导的。
英文摘要：
In this study, two enolase genes were isolated and characterized from the Chinese oak silkworm, Antheraea perny, which were designated as enolase Ⅰ and Ⅱ, respectively. The enolase Ⅰ cDNA sequence was 1712 bp with an open reading frame （ORF） of 1302 bp encoding 433 amino acids. The enolase Ⅱ cDNA sequence was 1549 bp with an ORF of 1296 bp encoding 431 amino acids. The amino acid sequences of the two genes share several conserved features/sites of enolase. Antheraea pernyi enolase Ⅰ shows 93%-97% sequence identity to enolases of lepidopterans available to date, 75%-82% identity to enolases of other invertebrates, 60%-72% identity to enolases of other organisms including vertebrates, plants, and fungi. Antheraeapernyi enolase Ⅱ shows 84% identity to Bombyx mori enolase Ⅱ, but 60% identity to A. pernyi enolase Ⅰ. In the phylogenetic tree, enolase Ⅱ sequences from A. pernyi and B. mori were clearly separated from the majority of enolase sequences of higher organisms including A. pernyi and B. mori enolase Ⅰ sequences. By sequence comparisons and phylogenetic analysis, we suggest that enolase Ⅱ from A. pernyi and B. mori may be a new member of the enolase superfamily. Antheraea pernyi enolase Ⅰ mRNA was found in all tested tissues whereas enolase Ⅱ mRNA was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase Ⅱ gene may be related to reproduction. The transcript abundance of A. pernyi enolase Ⅰ gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that A. pernyi enolase Ⅰ gene may be inducible by temperature stress.
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