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中文摘要：
以piggyBac转座子为介导的显微注射家蚕早期胚胎的技术，是目前获得转基因蚕的有效和主要方法。基于piggyBac以随机方式发生高频切出和转座的特点，已将其开发成各种遗传分析工具，用于果蝇、小鼠和家蚕等模式生物的基因功能研究。本文旨在分析外源piggyBac转座元件在转基因家蚕中的基因组整合位点特征，以期为更好的利用其开展家蚕转基因研究提供参考。采用反向PCR和生物信息学等方法，获得并分析了转基因家蚕中67个外源piggyBac的整合位点，结果显示：有28个整合位点位于基因间区，22个位于基因内部，但不在外显子区域；在整合位点两侧各10Kb区域范围内，注释基因多为转座酶或反转录酶等，推测其可能为piggyBac插入整合的热点区域（hotspotregions）；此外，有47个整合位点可定位至家蚕的18条染色体上。
英文摘要：
The technology of microinjecting preblastodermic eggs using a piggyBae transposon-de- rived vector is currently the most effective and main method to obtain the transgenic silkworm. Based on the characteristic of effective excision and insertion with a random mecha- nism, the piggyBac has been developed into various genetic tools and used to analyse gene function in model organisms such as fruit fly, mouse, silkworm and some others. We inves- tigated the insertion site of foreign piggyBac transposable element in the transgenic silk- worm, Bombyx mori, which might be helpful for the better use of piggyBac in silkworm transgenic research. Using the method of reverse PCR and bioinformatics, we obtained and analysed 67 insertion sites of foreign piggyBac from transgenic silkworm. The results showed that 28 piggyBac inserted into the intergenic region, 22 piggyBac inserted into the in- ternal region of gene, but not in exon. Further analysis of both upstream and downstream 10Kb regions from the insertion sites revealed that there were many genes such as trans- posase and retro-transposase, suggesting that these regions might be hot spot regions for in- sertion and integration of piggyBac. Moreover, 47 insertions have been mapped to 18 chromosomes in B. mori.
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