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中文摘要：
【目的】研究和检验利用Mascot程序与家蚕EST数据解析质谱分析的结果,鉴定目标蛋白的方法。【方法】将家蚕5龄起蚕中肠总蛋白进行二维电泳分离,并运用质谱对80个蛋白点进行肽指纹图谱分析,利用Mascot程序和经自主编制软件Transeq翻译的EST序列数据库对所得质谱数据进行鉴定。【结果】鉴定出其中41个蛋白,并利用EST电子克隆在其余39个蛋白中得到19个蛋白的全长或较长cDNA。【结论】本文提出的利用家蚕EST数据来解析家蚕蛋白质谱结果的方法,能够鉴定仅利用公共数据不能鉴定的蛋白,并能鉴别基因可变剪接后产生蛋白异形体的问题。
英文摘要：
【Objective】 Methodology for identification of silkworm（Bombyx mori） protein using mass spectra data,Mascot program and EST data was evaluated.【Method】 Total protein of the fifth instar silkworm midgut was separated by two-dimensional electrophoresis,and the peptide fingerprintings of 80 protein spots were analyzed using mass spectrometry.Those mass spectral data were identified using Mascot program and silkworm EST database translated by a software called Transeq.【Result】 Forty-one proteins were identified and the full-length or longer cDNA of 19 proteins among other 39 proteins were obtained using the EST in silico cloning.【Conclusion】 The methodology could be used to identify proteins using mass spectral data with silkworm EST data,as well as alternatively spliced variants.
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