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家蚕体形突变新缢蚕(co-n)的遗传分析与基因定位研究
  • 期刊名称:蚕业科学, 2009,35(1): 13-17
  • 时间:0
  • 分类:S882.6[农业科学—特种经济动物饲养;农业科学—畜牧兽医]
  • 作者机构:[1]西南大学生物技术学院,西南大学蚕学与系统生物学研究所,重庆400716, [2]重庆大学农学及生命科学研究院,重庆400030
  • 相关基金:国家自然科学基金项目(编号30671591),国家重点基础研究发展计划“973”项目(编号2005CB121000),国家重点高技术研究发展计划“863”项目(编号2006AA10A11-7),教育部博士点基金项目(编号20060635016),重庆市自然科学基金项目(编号CSTC2006BB1051),西南大学研究生科技创新基金项目(编号2006052).
  • 相关项目:家蚕新突变基因的发现、定位及遗传图谱的整合
中文摘要:

从西南大学家蚕基因库中发现体形自然突变新缢蚕。幼虫表型特征为:环节中央部位呈缢缩状,色素聚集,腹部节间膜呈隆起状,5龄中后期最明显,与缢蚕(constricted,co)相似。至成虫期观察,腹背通常少有鳞毛。经遗传分析,新缢蚕为隐性基因支配,将其命名为new constricted,基因符号:co-n。连锁分析结果表明,co-n与黄血(Y)基因连锁,故判定该基因属于第2连锁群。这是家蚕第2连锁群30多个突变中发现的第一个体形突变。经过新缢蚕(co-n)与灰色卵(Gr)、黄血(Y)基因间的三点测验定位实验,结合伴野丰2005年最新公布的家蚕连锁遗传图的第2连锁群基因座数据,将co-n基因座确定为:2-5.7。

英文摘要:

Abstract "New constricted" is a natural mutant of body shape discovered in the silkworm germplasm bank at Southwest University. Its larvae are characterized with constriction and pigment deposition in the center of body segments, and bulge at the abdominal inter-segmental membrane. These characters are especially obvious at later days of the fifth-instar, which make the larvae resemble those with the constriced (co) gene. Generally, the adults' dorsal and abdominal surfaces have very few scales. Genetic analysis showed that the new constricted appearance is controlled by a recessive mutant gene. It was named new constricted with the gene symbol co-n. Linkage analysis indicated that co-n is linked with yellow blood (Y) gene and belongs to the 2nd linkage group. It is the first body shape mutant among more than thirty mutants which have been discovered on the 2nd linkage group. The resutt of three-point test among new constricted (co-n), grey color egg (Gr) and yellow blood ( Y), and data of the latest linkage map published by Banno Yutaka in 2005 showed that the gene locus of co-n is at 2-5.7.

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