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中文摘要：
为了进一步认识家蚕浓核病毒BmDNV-3（中国株）VD1基因组的结构和功能，VD1被分离、纯化、克隆到pUC119载体上，完成了基因组全序列的测定。序列分析显示VD1基因组全长为6543个核苷酸，末端拥有224个核苷酸反向重复序列（ITRs）。VD1基因组正链含有3个大的开放阅读框（ORF1-3），负链含有1个大的开放阅读框（0RF4）。比较BmDNV-3的VD1和BmDNV-2（Yamanashi isolate）的VD1基因组全序列，两者同源性为98．4％，并且有107个碱基的替代和1个碱基插入，氨基酸突变集中在VD1ORF3和VD1ORF4。Northern杂交结果显示：VD1的左边正链上有1．1kb和1．5kb两个转录本，右边的负链上有一个3.3kb转录本。3′和5′-RACE结果显示：1．1kb转录本开始于nt290，结束于nt1437；1．5kb转录本开始于nt1423，结束于nt2931；3．3kb转录本开始于nt6287，结束于nt2922。正链上1．5kb转录本和负链上3．3kb转录本拥有10个核苷酸的3′端的共同序列。研究结果显示该病毒基因转录与已报道的其它浓核病毒存在较大的差异性。
英文摘要：
To better understand the genomic structure and function of Bombyx mori bidensovirus （China isolate） VD1 , the VD1 was purified and cloned into the pUC119 vector, and the complete nucleotide sequence of VD1 was determined. Sequence analysis showed that VD1 genome consisted of 6543 nts including inverted terminal repeats （ITRs） of 224 nts. In the viral genome, three major open reading frames （ORF1, ORF2 and ORF3） in the plus strand and one major ORF （ORF4） in the complementary strand were identified. Comparison of the complete genome sequence between Bombyx mori bidensovirus（ China isolate） and BmDNV-2 （Yamanashi isolate） showed an identity of 98.4% in VD1 , with a total number of 104 bp substitutions and 1 bp insertions found in Bombyx mori bidensovirus（China isolate）, the highly variable regions were mainly located in VD1 ORF3 and VD10RF4. Northern blotting revealed that VD1 contained 1.1 kb and 1.5 kb transcript in the left-half ‘plus＇ strand, and one transcript about 3.3 kb of ‘minus＇ strand in the right-half. Sequencing of 3＇ and 5＇ ends of transcript products showed the 1.1 kb transcript started at nt 290 and ended at nt 1437, the 1.5 kb transcript was found to start nt 1423 and ended at 2931, and the 3.3 kb transcript was found to start nt 6287 and ended at nt 2922. Therefore, the 1.5 kb transcript in the left-half plus＇ strand and 3.3 kb transcripts of minus＇ strand in the right-half overlapped for 10 nts at the 3＇ ends. These results indicate that this virus employs a transcription strategy that is radically different from that of the other reported DNVs.
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