以‘八月红’ב砀山酥梨’的97株F1群体为试材,利用AFLP技术构建梨(2n=34)的分子遗传连锁图谱,共获得17个连锁群,包含209个标记位点,覆盖基因组1506.3cM,平均图距为7.21cM;用区间作图法进行梨果实可溶性固形物、单果质量、果实纵径、果实横径和纵横径比等5个性状的QTL定位,其中在LG1连锁群上定位可溶性固形物含量QTL1个;在LG6、LG7和LG11连锁群上定位单果质量QTL各1个;在LG7连锁群上定位果实横径的QTL1个;在LG6和LG7连锁群上定位果实纵径的QTL各1个;在LG1和LG8连锁群上定位果形指数的QTL各1个。各QTL位点的LOD值在2.50~4.14之间,可解释11.4%~36.4%的表型变异。
A genetic linkage map of Pyrus(2n=34)was constructed by 209 AFLP markers which were distributed on 17 linkage groups and spanned 1 506.3 cM in genome with an average distance of 7.21 cM between markers.The map was based on a population(n=97)derived from a cross of'Bayuehong'× 'Dangshansuli'.By using interval mapping method,the QTLs for five fruit traits of pear were detected,including 1 QTL for soluble solids content on LG1,3 QTLs for fruit weight on LG6,LG7 and LG11,1 QTL for aspect ratio on LG7,2 QTLs for fruit weight on LG6 and LG7,and 2 QTLs for fruit stalk length on LG1 and LG8.The phenotypic variations explained by each QTL ranged from 11.4%to 36.4%,and their LOD values varied from 2.50 to 4.14.