以设施栽培的3 a生藤稔葡萄品种嫁接苗(贝达砧木)为试材,研究了硒的不同应用方法和剂量下葡萄不同器官对硒的积累和果实品质的影响。结果表明,(1)盛花期土施0.2 g.株-1 Na2SeO3后,设施葡萄的叶片、叶柄与果肉硒含量及单果硒总量从花后至成熟均呈"升-降"变化趋势。(2)与对照相比,土施0.2 g.株-1 Na2SeO3后,不仅叶片、叶柄和果肉中的硒含量显著增加,而且硒的累积顺 序也发生变化,变为果肉〉叶片〉叶柄。(3)硒元素在设施葡萄果实中的积累与分布因施用方式不同而异。土施硒肥,硒在果实中优先累积到种子中,其次累积到果肉中,最后累积到果皮中;而叶面喷施硒肥,硒在果实中优先累积到果皮中,其次累积到果肉中,最后才累积到种子中。(4)土施Na2SeO3以幼果发育期施用效果较好;设施葡萄果实各部位硒含量与外源硒施入量呈显著正相关;同时随外源 硒施入量的增加,设施葡萄果实可溶性固形物含量随之升高,但当外源硒施入量过大时,设施葡萄果实可溶性固形物反而下降。
Three-year-old grapevine cv Fujiminori, grafted on Beta rootstock, were planted in the experimental orchard of Fruit Research Institute, Chinese Academy of Agricultural Sciences in order to study Selenium absorbtion, distribution and accumulation in the protected cultivated grapevine and the effect of Se on the fruit qualities. The results indicated that after treatment with 0.2 g. plant-1 NazSeO3 at flowering, Se concentration in leaves, leafstalks, berry flesh and in single beery from flowering to fruit ripening firstly rose and then dropped. Se concentration in leaves, leafstalks and berry flesh increased significantly compared with control and the orders of Se distribution changed to berry flesh 〉leaf〉leafstalk after treatment. The orders of Se distribution in the fruit were seed〉berry flesh〉skin with soil application, and skin〉 berry flesh〉seed with spraying selenium solution. Se concentration in berry flesh with soil application at 20 to 35 days after flowering was more than that at other berry development stages. Se concentration in analyzed parts of berry was significantly corresponded with the increase of selenium doses . TSS rose significantly after Se application and reached the highest with 0.15 g·fruit-l Na2SeO3, but dropped with 0.2 g·plant-I Na_2SeO_3.