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中文摘要：
为探讨日粮添加碳酸氢钠（NaHCO，）对蛋壳质量、蛋壳钙、血钙浓度、钙调激素水平以及蛋壳腺中ca“转移相关蛋白基因表达的影响，选取900只2l周龄海兰褐蛋鸡随机分为对照组和1、5、25和50g·kg-1 NaHCO3添加组，每组6个重复，每个重复30只，持续10周。结果表明：与对照组相比，Et粮添加1和5g·kg-1NaHCO，对蛋壳质量不产生显著影响（P〉0．05），25和50g·kg。组蛋壳钙含量显著降低（P〈0．01），蛋壳质量、厚度降低（P〉O．05），50g kg-1组蛋壳强度和血清Ca2＋浓度亦显著降低（P〈0．05）。此外，随着NaHCO，添加量的增加，25和50g·kg-1组血液维生素D水平显著或极显著增加（P〈O．05或P〈O．01），蛋壳腺中瞬时性受体电位通道香草精受体6（TRPV6）、钙结合蛋白（CaBP—D28k）、钠-钙交换蛋白（PMCA1b）和质膜钙离子ATP酶通道（NCXl）mRNA表达量显著提高（P〈0．05），50g·kg-1。组碳酸酐酶（CA）mRNA水平显著降低（P〈O．05）。结论：高剂量的NaHCO，（≥25g·kg-1）使蛋壳质量下降，CAmRNA表达量降低，致使蛋壳腺Ca2＋转移受阻，蛋壳钙含量减少、质量下降。其次，低血钙和高维生素D水平使TRPV6、CaBP—D28k、PMCAlb和NCXI等基因表达增加，表明钙离子跨细胞转运途径在蛋壳腺中起补充转移作用。
英文摘要：
The purpose of this study was to analyze the effect of dietary NaHCO3 on eggshell quality, the concentration of Ca2~ in eggshell and plasma, and levels of plasma calcium regulating hormones, as well .as mRNA expression of genes related to Ca2＋ transport in eggshell gland（ESG）. Nine hundred 21-weeks-old Hy-line brown laying hens were randomly assigned to be fed with 0,1,5,25 and 50 g.kg-1 NaHCO3 for 10 weeks,and each group was composed of 6 replicates and 30 birds per replicate. The results showed as follows ：compared with control,the eggshell quality was not influenced by dietary NaHCO3 level at 1 g kg-1 or 5 g kg-1（ P〉0.05 ）. However,the content of Ca2＋ in eggshell（P〈0.01 ）, eggshell thickness and weight（ P〉0.05 ）were reduced in 25 g. kg-1 and 50 g kg-1 groups ,respectively. In addition ,eggshell strength and plasma Ca2＋ content were also significantly reduced by the supplementation of 50 g kg-1 NaHCO3 （P〈0.05）. Then, the concentration of vitamin D level in plasma was dramatically increased by dietary NaHCO3 level at 25 and 50 g＇kg-t（P〈O. 05 or P〈0.01 ）. Moreover,the mRNA expression of transient receptor potential vanilloid receptor 6（TRPV6）, calbindin-D28k（CaBP-D28k）, plasma membrane Ca2＋-ATPase （PMCA lb）arid Na＋/Ca2＋ exchangers （NCX1）in ESG tissue were increased in 25 and 50 g kg-1 NaHCO3 groups（ P〈0.05 ）,but the level of carbonic anhydrase（CA） mRNA was remarkably reduced in 50 g kg-1 group（P〈0. 05）. In conclusion： the mechanism was that lower plasrrta Ca2＋ content and CA mRNA level were induced by excessive NaHCOd（ t〉25 g.kg 1 ） ,then which led to block to Ca2＋ transfer in ESG,finally resulted in the reduction of eggshell quality. Secondly,the increase in mRNA expression of TRPV6, CaBP-D28k, PMCA l b, and NCX1 suggests that transcellular Ca2＋ transport pathway appears to compensate for low Ca2＋ intake in ESG.
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