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中文摘要：
以含不同浓度黄曲霉毒素B1（AFB1）的配合饲料饲喂异育银鲫（Carassius auratus gibelio）成鱼56d,研究异育银鲫成鱼[（122.3±0.7）g]生长、生理反应、肝脏组织学变化、卵巢发育以及鱼体各组织中的AFB1的毒素积累状况。实验分为5个实验组,不同实验组饲料中AFB1含量分别为0、5、20、50、500μg/kg饲料（实测值分别为2.59、4.12、12.39、46.23、454.07μg/kg饲料）,每个处理3个平行。在整个实验过程中各实验组均未表现出外部形态和行为异常,各组存活率均达到100%。各实验组异育银鲫成鱼终末体重、摄食率（FR）、特定生长率（SGR）和饲料效率（FE）均无显著差异。饲料AFB1水平对异育银鲫血清总胆固醇（TC）含量、血清谷丙转氨酶（GPT）、谷草转氨酶（GOT）和碱性磷酸酶（AKP）活性均无显著影响。各毒素组血清超氧化物岐化酶（SOD）活性与对照无显著差异。各毒素组肝脏和卵巢均未见明显的组织学病理变化。肌肉和性腺中的AFB1积累量低于FDA食品安全限定标准（5μg/kg）。肝胰脏中的AFB1积累和饲料中的AFB1水平呈对数关系。饲喂AFB1≥50μg/kg饲料使异育银鲫成鱼肝脏AFB1积累超过安全限量标准。结果表明,异育银鲫成鱼至少可耐受AFB1含量达500μg/kg饲料（实测值：454.07μg/kg饲料）56d。
英文摘要：
A 56-day feeding trial was conducted to evaluate the effect of dietary aflatoxin B1（AFB1） on growth,physiological responses,histological changes,and accumulation in gibel carp（Carassius auratus gibelio） adult.Triplicate groups of gibel carp [（122.3 ± 0.7） g] were fed with five semi-purified diets（Diet 1 to 5） containing0,5,20,50 and 500 μg/kg AFB（determined level was 2.59,4.12,12.39,46.23 and 454.07 μg AFB1/kg diet,respectively）.During the experiment,photoperiod was 12D∶12L with the light period from 09：00 to 21：00,dissolved oxygen was above 6 mg/L,ammonia-N was less than 0.4 mg/L and pH was about 6.8.The results showed that during the 56-day of AFB1 exposure,no external changes and unusual behavior were observed in the fish fed with various levels of AFB1.The survival rate in all groups attained 100%.There was no significant difference in final body weight,feeding rate（FR）,specific growth rate（SGR） and feed efficiency（FE） between the control and the other groups.Fish fed with various levels of AFB1 showed no significant differences in total cholesterol,activities of serum alanine aminotransferase（ALT）,asparitic aminotransferase（AST）,alkaline phosphatase（ALP） and superoxide dismutase（SOD） compared to the control group.No significant histological lesions in hepatopancreasand ovary were identified between the control and increasing AFB1 treatments.Low AFB1 residues were found in muscles and ovary,and were below the safety limitation of 5 μg/kg.Accumulation of AFB1 in hepatopancreas was logarithmically correlated to the dietary AFB1 level.Our results indicated that gibel carp was a less susceptible species to AFB1 exposure up to approximately 500 μg/kg diet（determined level was 454.07 μg/kg diet）,at least for 56 days.
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