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中文摘要：
目的：观察HCV核心蛋白对低侵袭性人肝癌细胞系SMMC-7721增殖的影响。方法：将HCV核心蛋白的原核表达质粒pcDNA3.0-C-EGFP转染SMMC-7721细胞,以空质粒pcDNA3.0（-）转染的细胞作对照。分别采用RT-PCR、Western blot和ELISA法检测转染细胞HCV核心蛋白mRNA、蛋白及培养上清中蛋白的表达水平;进行纤维连接蛋白黏附实验、侵袭实验及运动实验,MTT法观察细胞增殖情况。结果：重组质粒pcDNA3.0-C-EGFP转染的SMMC-7721细胞HCV核心蛋白mRNA、蛋白及培养上清中蛋白的表达水平,细胞黏附率,侵袭实验及运动实验穿膜细胞数均高于对照（t=8.141、5.762、19.931、2.961、4.112和4.213,P均〈0.05）。pcDNA3.0-C-EGFP转染的SMMC-7721细胞增殖率也较对照明显增加（P〈0.05）。结论：HCV核心蛋白外源基因可促进SMMC-7721细胞系的增殖
英文摘要：
Aim ： To investigate the effect of HCV core protein on the proliferation of human hepatocellular carcinoma cell line SMMC-7721. Methods ：SMMC-7721 cells were transfected with plasmid pcDNA3.0-C-EGFP,and cells transfected with peDNA3.0 （ - ） served as controls. The mRNA expression in the cells, and protein expressions in the cells and in the supernatant of HCV core protein were verified by RT-PCR,Western blot and ELISA methods,respectively. A series of func- tional assays were used to monitor the changes of the cells adhesive, migrant and invasive capabilities,and MTT method was used to detect cell proliferation. Results： The mRNA expression in the cells, and protein expressions in the cells and in the supernatant of HCV core protein, adhesive, migrant and invasive capabilities of the cells transfected with pcDNA 3.0-C- EGFP were higher than those of the control （ t = 8.141,5. 762, 19.931,2.961,4.213, and 4.112, P 〈 0.05 ）. The prolifera- tion rate of the cells transfected with pcDNA3. O-C-EGFP was higher than that of the control（P 〈 O. 05）. Cotlclttsiorl：HCV core protein might enhance the proliferation of SMMC-7721 cells.
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