中文摘要：

为了研究不同剂量赭曲霉毒素A（OTA）廿玉米赤霉烯醇（α-ZOL）联合作用对HepG2细胞毒性的交互作用及其可能机制，试验采用3×3析因试验设计，设OTA浓度和α-ZOL浓度2个因素，OTA浓度水平分别为O、6和12gmol／L，α-ZOL浓度水平分别为0、15和30μmol／L，全组合后共组成9个水平组合．毒素作用时间为48h．用边际均数轮廓图判断细胞毒性和氧化损伤的交互作用，结果发现OTA和α-ZOL联合作用时，细胞毒性、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、谷胱甘肽过氧化物酶（GSH—PX）活性和谷胱甘肽（GSH）含量的交互作用一致，均表现为拮抗效应；且细胞活力的变化与SOD活性、MDA含量、GSH—PX活性和GSH含量的变化表现为显著性相关（P〈0．05）．结果表明细胞毒性的交互作用为拮抗效应，且氧化损伤是毒素诱发细胞毒性的主要途径之一．

英文摘要：

The objectives of this study were to investigate the combined effects of ochratoxin A （OTA） and a-zearalenol （a-ZOL） on Hep G2 cells, and to explore the cytotoxicity mechanism. 3 X 3 factorial analy- sis design was applied in this trial. Nine groups were adopted to investigate the combined cytotoxicity of three levels of OTA （0/lmol/L, 6μmol/L and 12μmol/L） and three levels of a-ZOL （0μmol/L, 15/lmol/L and 30μmol/L） after 48 h of exposure. The cytotoxicity of two combined mycotoxins was evaluated by u- sing the estimated marginal means. The results demonstrated the cytotoxicity of two combined mycotoxins indicated an antagonism. And the activities of intracellular SOD and GSH-PX, the content of intracellular MDA and GSH indicated an antagonism. There was a significant correlation between the cytotoxicity and the activities of intracellular SOD and GSH-PX （P〈0.05）, and there was a significant correlation between the cytotoxiciny and the content of intracellular MDA and GSH （P〈0. 05）. The results suggest that the combined cytotoxicity show an antagonism,and oxidative damage is one of the pathway of the cytotoxicity.