中文摘要：

建立了超滤浓缩-高效液相色谱法测定乳中乳铁蛋白的方法。该方法样品处理为样品用磷酸盐缓冲液稀释后，样液先通过肝素免疫亲和柱净化、磷酸氢二钠-氯化钠溶液洗脱，然后采用超滤浓缩后直接测定。采用紫外检测器检测。本方法检出限为0．01mg／100g。该法具有样品处理简单方便，灵敏度高，重现性好，分析时间短等优点。该方法测定的为具有活性功能的乳铁蛋白含量，解决了乳铁蛋白质量分数较低的生乳及各类液态乳只采用肝素亲和柱净化后测定灵敏度不能满足测定要求的问题，对评价乳的营养价值提供准确数据。

英文摘要：

To establish a method for determination of lactoferrin in milk by ultrafiltration and HPLC. The method of sample treatment for samples with phosphate buffer diluted sample solution by heparin immunoaffinity column, two sodium hydrogen phosphate Sodium Chloride Solution elution, then using ultrafiltration after direct determination by UV detector, the detection limit of the method is simple and convenient, this method has high sensitivity for 0.01 mg/100g, good reproducibility, short analysis time. The method for the determination oflactoferrin has active function, solves all kinds of raw milk and liquid milk lactoferrin mass fraction low production only The determination of the sensitivity of the heparin affinity column after purification can not meet the requirements of the measurement, and provide accurate data for evaluating the nutritional value of milk.