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中文摘要：
为研究运输应激对脑内五羟色胺（5-hydroxytryptamine,5-HT）表达的影响及酸枣仁散对运输应激的调控作用,试验采用摇床模拟公路运输途中的摇晃、高温和拥挤等因素,建立大鼠运输应激模型（35℃,60r/min,每天应激2h,连续7d）。将40只大鼠随机分为对照组、酸枣仁组、应激组和应激＋酸枣仁组,分别处理7d后,断头取脑,利用酶联免疫吸附法（ELISA）和免疫组化法检测各组大鼠脑区5-HT含量变化及分布。酶联免疫吸附法检测结果显示,与对照组相比,应激组大鼠中缝核、海马和纹状体等脑区5-HT表达量均明显减少（P〈0.05）,应激＋酸枣仁组较应激组5-HT表达量显著增加（P〈0.05）。免疫组化结果显示,在中缝背核及其亚核DRDsh、DRv和DRc亚核,应激组大鼠5-HT灰度值与对照组相比显著下降（P〈0.05）,应激＋酸枣仁组与应激组相比显著上升（P〈0.05）。以上结果表明,运输应激可以导致中枢神经系统部分脑区5-HT含量下降,而酸枣仁散可能通过上调脑内5-HT的含量来调控运输应激。
英文摘要：
In order to investigate the impact of transport stress on 5-HT in rat brain and the regulation of Suanzaoren San（named as SZR）,a swing bed was used as transport stress module（named as TS）under condition of 35 ℃,60 rpm and 2h/d for 7days.Forty rats were divided randomly into four groups：the control group,the SZR group,the TS group and the SZR＋TS group.The brain tissues were taken after 7days treatment.The level of 5-HT in different regions of the brain were detected using enzyme-linked immunosorbent assay（ELISA）.The distribution and quantity of serotonergic neurons were also examined by immunohistochemical technique.The results of ELISA showed that the content of 5-HT was significantly decreased（P〈0.05）in dorsal raphe nucleus,hippocampus and striatum after transport stress.Moreover,5-HT content in the SZR＋TS group was significantly increased（P〈0.05）in these regions,compared with the TS group.The immunohistochemical results showed that 5-HT expression in the TS group was decreased significantly compared to control group in DRDsh,DRv and DRc（P〈0.05）.While the level of 5-HT in the TS＋SZT group was increased compared with the TS group.In conclusion,transport stress can decrease the level of 5-HT in different brain regions,and SZR could regulate transport stress by increasing the level of 5-HT.
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