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牛A型多杀性巴氏杆菌分离株的鉴定和免疫原性研究

ISSN号：0529-6005
期刊名称：《中国兽医杂志》
时间：0
分类：S855.12[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]华中农业大学农业微生物学国家重点实验室,湖北武汉430070, [2]华中农业大学动物医学院,湖北武汉430070, [3]华中农业大学动物科技学院,湖北武汉430070, [4]武汉科前动物生物制品有限公司,湖北武汉430070
相关基金：农业公益性行业科研专项（201003060）;现代农业（肉牛/牦牛）产业技术体系专项经费资助（CARS-38）

作者：汪漫[1,2], 胡长敏[2], 陈颖钰[3], 彭清洁, 姜鹏[2], 崔鹏[1,2], 陈焕春[1,2], 郭爱珍[1,2]

关键词：巴氏杆菌, A型多杀性巴氏杆菌, 疫苗, 毒力, 免疫, 牛, Pasteurella, capsular serotype A Pasteurella mutocida, vaccine, virulence, immunization, cattle

中文摘要：

从湖北黄冈、洪湖、钟祥和河南驻马店发病肉牛场送检病变肺中分离到4株病原菌,经过菌落形态观察、培养特性、生化反应、PCR鉴定等检测,确定这4株临床分离株均为荚膜A型多杀性巴氏杆菌,分别命名为Pm-HG、Pm-HH、Pm-ZX和Pm-ZMD.比较4株临床分离株与参考株的生长特性,发现生长曲线与参考株无明显差异.小鼠毒力试验表明,Pm-HG、Pm-HH和Pm-ZX接种10个菌以下即可将小鼠致死,毒力均很强.将4株菌灭活后免疫小鼠,二免后2周,血清抗体效价都在1∶20 000以上,其中Pm-ZMD和Pm-HG抗体效价最高.用各株菌进行交叉攻击,结果Pm-HG的交叉保护率最高,除能完全保护同种菌的攻击外,对其他3株菌的交叉保护率都在90％以上.因此Pm-HG可望作为进一步研发A型多杀性巴氏杆菌灭活疫苗的制苗菌株.

英文摘要：

Four clinical isolates of capsular serotype A Pasteurella mutocida, designated as Pm-HG, Pm-HH, and Pm-ZX, and Pm-ZMD respectively, were isolated from lesioned lung tissues of cattle at Huanggang, Honghu, and Zhongxiang in Hubei,and Zhumadian in Henan. The culture characteristics and bacterial stain, biochemistry test and PCR detection of specific genes were examined to characterize the iso-lates. The growth curves of these four isolates were found to he similar to the reference strain. The patho-genicity test in mice showed that Pm-HG, Pm-HH and Pm-ZX have a stronger virulence. The isolates were killed by formalin as the immunogen and emulsified with mineral oil to make the inactivated vaccines and inoculated into mice. At week 2 after 2 inoculations, the titers of serum antibody reached 1 ： 20 000 for all groups with Pm-ZMD and Pm-HG the highest. The cross-challenge of the immunized mice was con-ducted with the 4 virulent strains and results showed that Pm-HG had the highest rate of cross-protection. It provided a complete protection against the self challenge, and meanwhile, the cross protection rates for the attack of other three strains were above 90%. Therefore the strain Pm-HG could be taken as an ideal stain for development of potential inactivated vaccine against P. rnutocida.

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