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中文摘要：
为制备牛腺病毒3型（BAV-3）的单克隆抗体（MAb），本研究采用原核表达系统截短表达了BAV-3六邻体蛋白（Hexon），并以纯化的重组蛋白免疫BALB/c小鼠，经细胞融合筛选出14株杂交瘤细胞株。Western blot与间接免疫荧光试验表明14株MAbs均具有良好的反应性。特异性试验表明这些MAbs与牛副流感病毒3型、牛病毒性腹泻病毒及牛传染性鼻气管炎病毒无交叉反应。免疫组化试验表明其中一株1F8亲和力最强，可以用于检测BAV-3。本研究为进一步鉴定BAV-3抗原表位及研发相应的诊断试剂奠定了基础。
英文摘要：
Bovine adenovirus type 3 （BAV-3） is considered one of the most important respiratory tract agents of cattle diseases. To prepare monoclonal antibodies （MAbs） against Hexon protein of BAV-3, the SP2/0 cells were fused with the splenic cells from BALB/c mice immunized with truncated Hexon protein expressed in E.coli and 14 hybridomas secreted MAbs against BAV-3 were obtained after screening by indirect ELISA based on BAV-3. All the MAbs were positively reacted with BAV-3 detected by western blot and indirect immunofluorescence assay. Further specific assays indicated that 14 MAbs were specific to BAV-3, but not cross-reacted with bovine parainfluenza virus type 3, bovine viral diarrhea virus and bovine herpesvirus type 1 by ELISA assays. In addition, the MAb 1F8 had the higher antibody affinity which was suitable for detecting BAV-3 in animal tissue sample by immunohistochemistry test. The preparation of the MAbs provided the basis for fiLrther epitope identifications of BAV-3 hexon protein and development of diagnostic methods for BAV-3 infection.
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