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中文摘要：
目的：探讨pIL-12质粒DNA免疫对细粒棘球蚴感染小鼠脾细胞凋亡的影响。方法：将pIL-12质粒DNA肌肉注射免疫BALB／C鼠，免疫后8周用Eg原头节进行攻击感染，感染后18周剖杀小鼠，计算减蚴率；取脾并分离脾细胞，用ConA刺激培养脾细胞，流式细胞仪检测脾细胞的凋亡发生率，同时设有BCG和PBS对照。结果：pIL-12质粒DNA免疫组的减蚴率为44．85％，脾细胞凋亡发生率明显低于PBS对照组。结论：pIL-12质粒DNA可抑制细粒棘球蚴感染小鼠的脾细胞凋亡。
英文摘要：
Objective： To investigate the effects of pIL-12 plasmid DNA on apoptosis of splenocytes in mice infected with protoscoleces of hydatid cyst of E.granulosus （Eg）. Methods： BALB/c mice were intramuscularly vaccinated by pIL-12 plasmid DNA, then challenged with Eg protoscoleces on 8 w of vaccination and killed on 18W of infection to count rate of reduced hydatid cyst weight.The spleen was gathered to separate splenocytes, splenocytes then were cultured by stimulation with ConA, and apoptotic rate of splenocytes were measured by FACsort.BCG and PBS were served as control. Results： In the group of pIL-12 plasmid DNA immunization, the rate of reduced hydatid cyst weight was 44.85% ;apoptotic rate of splenocytes in the pIL-12 plasmid DNA group was lower than that in the PBS control.Conclusion： pIL-12 plasmid DNA may inhibit apoptosis of splenocytes in mice infected with hydatid cyst of Echinococcus granulosus.
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