中文摘要：

俘虏人口为再介绍为危及的动物和个人的来源提供一个宝贵基因资源给弄空的产地。因此，在决定渊源的精确性具有为管理俘虏人口并且在为版本选择已知的基因特征的代表性的个人的重要重要性。在这研究，我们建立了一个快、有效的方法进行渊源在 Wolong 人口为俘虏巨人熊猫测试。这种技术开始使用二高度多态的 microsatellites，五个不太多态的标记的随后的使用然后测试的渊源排除用我们开发了的巨大的熊猫渊源排除节目执行了。我们的结果表明(1 ) 标记的两个集合成功地在渊源测试的 25 种情况中识别了真正的父亲，(2 ) 渊源排除的成功率与使用的标记的多型性的度变化了。随后，我们与这些标记进行了在渊源鉴定的成功率之间的关联分析，基因差异的参数和中性的测试。我们发现 microsatellites 的渊源排除力量显著地与等位基因(Na ) 的数字被相关，期望杂合现象(H E ) 并且观察了 homozygosity 统计数值(F O )( 所有 P < 0.05 ) 。从这，我们开发了一个新变量， Na ?????????? à

英文摘要：

Captive populations provide a precious genetic resource for endangered animals and a source of individuals for reintroduction to depleted habitats. Therefore, accuracy in determining paternity is of vital importance for managing captive populations and in selecting representative individuals of known genetic characteristics for release. In this study, we established a fast and effective method to conduct paternity testing for captive giant pandas in the Wolong population. This technique uses two highly polymor- phic microsateUites initially, subsequent use of five less polymorphic markers and then paternity exclusion testing carded out using the giant panda paternity exclusion program we have developed. Our results revealed that （1） both sets of markers success- fully identified the real fathers in 25 cases of paternity testing and （2） the success rate of paternity exclusion varied with the de- gree of polymorphism of the markers used. Subsequently, we conducted correlation analysis between the success rates of paternity identification with these markers, parameters of genetic diversity and tests of neutrality. We found that the paternity exclusion power of microsateUites was significantly correlated with the number of alleles （Na）, expected heterozygosity （HE） and observed homozygosity statistic （Fo） （all P 〈 0.05）. From this, we developed a new variable, NaXHE/Fo, showing a highly significant positive correlation with the resolution power of microsatellites （P = 0.001）. Moreover, the first two highly polymorphic loci gave a 100% success rate of excluding non-paternal males because they yielded higher values of NaxHE/Fo than the other five less polymorphic markers. Thus, the NaxHE/Fo parameter appears suitable to serve as a criterion for selecting microsatellite markers, which could be used for high-resolution molecular techniques of paternity determination among a range of captive animals besides giant pandas.