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Molecular identification and phylosenetic position of Cuora yunnanensis

ISSN号：1005-0094
期刊名称：《生物多样性》
时间：0
分类：Q7[生物学—分子生物学]
作者机构：[1]State Key Laboratory of Genetic Resource and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China, [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China, [3]Department of Systematics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China, [4]Nanjing Museum of Turtles and Tortoises, Nanjing 210029, China
相关基金：Supported by the National Basic Research Program of China （973 Program）（Grant No. 2007CB411600）, the National Natural Science Foundation of China （Grant No. 30621092）, and Bureau of Science and Technology of Yunnan Province, China 1） Iverson J B. A revised checklist with distribution maps of the turtles of the world. 1992, Privately printed. Richmond

作者： HE Jing[1,2], ZHOU Ting[4], RAO DingQi[3], ZHANG YaPing[1]

关键词：分子辨别, 线粒体, DNA, 生物学, Cuora yunnanensis, mitochondria DNA, conservation

中文摘要：

云南盒子鳖(Cuora yunnanensis， Bouienger， 1906 ) ，它在保存生物学引起了许多注意，自从它以前仅仅从在云南收集的 12 个标本被知道，被认为是绝灭，中国，在 1908 前。最近，实时标本被发现了它被建议是 C。yunnanensis。决定最新发现的标本是否确实是 C。yunnanensis，我们建立了分子的 phytogeny，与线粒体的 1725-bp 碎片！直接存储器存取，用从 C 的三个实时个人的样品。yunnanensis，和从 C 的一个博物馆标本的顺序数据。yunnanensis (MNHN 1907.10 ) 和类 Cuora 的另外的成员。我们发现三个最新发现的个人和 C.yunnanensis 的旧博物馆标本在形态学并且在 mitochondrial 直接存储器存取顺序是很类似的，建议三个新个人是 C。yunnanensis，并且这样种类不是绝灭的。我们的种系发生的分析也表明那 C。yunnanensis 不具有最近的混合起源，但是相当代表一个不同的进化的系。

英文摘要：

The Yunnan box turtle （Cuora yunnanensis, Boulenger, 1906）, which has drawn much attention in conservation biology, was regarded as extinct since it was previously known only from 12 specimens collected in Yunnan, China, before 1908. Recently, live specimens have been discovered which are suggested to be C. yunnanensis. To determine whether the newly discovered specimens are really C. yunnanensis, we have established a molecular phylogeny, with a 1725-bp fragment of mitochondrial DNA, using samples from three live individuals of C. yunnanensis, together with sequence data from a museum specimen of C. yunnanensis （MNHN 1907.10） and other members of the genus Cuora. We found that the three newly discovered individuals and the old museum specimen of C. yunnanensis are very similar both in morphology and in mitochondrial DNA sequence, suggesting that the three new individuals are the very C. yunnanensis, and thus the species is not extinct. Our phylogenetic analysis also demonstrates that C. yunnanensis is not of recent hybrid origin, but rather represents a distinct evolutionary lineage.