中文摘要：

目的：建立同时测定麻杏石甘汤中麻黄碱、伪麻黄碱、苦杏仁苷、甘草苷和甘草酸5种成分含量的高效液相色谱法。方法：采用Agilent ZORBAX SB-Aq C18柱,流动相为乙腈-0.1%磷酸梯度洗脱,柱温为30℃,于207nm波长处测定麻黄碱、伪麻黄碱和苦杏仁苷,237nm处测定甘草苷和甘草酸,流速为1m L/min。结果：麻黄碱、伪麻黄碱、苦杏仁苷、甘草苷和甘草酸分别在24.9-498.5、10.0-199.8、107.3-2 145.0、11.0-243.3、32.5-698.3μg/m L浓度范围内线性关系良好（相关系数均大于0.999）,5种成分的方法精密度（RSD）分别为1.40%、0.86%、1.49%、2.14%、1.20%,平均加样回收率分别为98.88%、99.03%、98.18%、97.31%、97.78%。结论：本法可同时测定麻杏石甘汤中上述5种成分,方法简便、重现性好,可用于麻杏石甘汤及其制剂的质量控制。

英文摘要：

Objective： To establish the method for simultaneous determination of ephedrine, pseudoephedrine, amygdalin, liquiritin and glycyrrhizic acid in Maxing Shigan Decoction by HPLC. Methods： The Agilent ZORBAX SB-Aq C18 column maintained at 30 oC with a gradient mobile phase system consisting of ACN-0.1% phosphoric acid solution was employed for sample analysis. The wavelength of the UV detector was set at 207 nm for ephedrine, pseudoephedrine and amygdalin determination, whilst 237 nm for liquiritin and glycyrrhizic acid determination with a flow rate of 1m L/min. Results： The linear ranges of ephedrine, pseudoephedrine, amygdalin, liquiritin and glycyrrhizic acid were 24.9-498.5, 10.0-199.8, 107.3-2145.0, 11.0-243.3, 32.5-698.3μg/m L, respectively（r0.999）. The precisions of the method was 1.40%, 0.86%, 1.49%, 2.14%, and 1.20%, whilst the average recoveries was 98.88%, 99.03%, 98.18%, 97.31% and 97.78%, respectively. Conclusion： The method is convenient with a good reproducibility and could determine 5 active ingredients of Maxing Shigan Decoction, which could be used as a quantitative analysis for Maxing Shigan Decoction and its preparations.