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中文摘要：
以蛇皮果顶梢嫩叶为材料,比较了CTAB法、改良CTAB法、CTAB-free法和SDS法对基因组DNA的提取效果,并对蛇皮果RAPD反应体系进行优化。结果表明,CTAB法、改良CTAB法和CTAB-free法均能提取出蛇皮果基因组DNA,其中NaCl浓度为5 m ol/L的改良CTAB法因可更有效地去除多糖及酚类物质,并能满足PCR扩增的要求而被认为是蛇皮果基因组DNA提取的最适方法;在15μL的PCR扩增体系中,Mg2＋、dNTPs和Taq DNA聚合酶的最优浓度分别为1.4 mmol/L、0.12 mmol/L和0.12 U/μL;PC R反应程序的最适退火温度为39℃。
英文摘要：
By taking the tender leaves on top-shoots of Salacca edulis as tested materials, the extraction effects of CTAB, improved CTAB, CTAB-free and SDS on the genomic DNA of salak were investigated and compared. And the reaction system of salak RAPD was optimized. The results show that CTAB, improved CTAB, CTAB-free all could extracted out genomic DNA from salak, of them, the improved CTAB with NaC1 concentration of 5 mol/L could more effectively remove polysaccharides and polyphenols than the other two methods, and meet the requirement of PCR amplification, so it is the best method. The optimal concentrations of Mg2＋, dNTPs, Taq DNA polymerase and the annealing temperature are 1.4 mmol/L, 0.12 mmol/L, 0.12 U/μL and 39 ℃ respectively for the PCR progress for salak.
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