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中文摘要：
B型烟粉虱Bemisia tabaci（Gennadius）biotype B和温室白粉虱Trialeurodes vaporariorum均为全球普遍发生的重要害虫。本研究以其他昆虫热激蛋白90基因（hsp90）保守区域设计兼并引物扩增两种粉虱hsp90中间片段，然后利用RACE技术获得全长cDNA。温室白粉虱却9D全长cDNA的开放性阅读框2166bp，编码722个氨基酸；烟粉虱hsp90全长cDNA的开放性阅读框2160hp，编码720个氨基酸。两种粉虱HSP90的完整氨基酸序列相似性高达92．94％，并均具有定义HsPg0家族签名序列的5个氨基酸保守区域和末尾基序“MEEVD”。通过real-timePCR技术，探测到两个基因在mRNA水平上皆能高温诱导表达。采用昆虫纲所有完整HSP90氨基酸序列进行Kimura双参数遗传距离分析并构建NJ进化树，结果显示hsp90在昆虫纲低级阶元水平和高级阶元水平系统进化上能得到一个较理想结果。本研究结果为B型烟粉虱和温室白粉虱抗逆适应性研究提供基础，并进一步验证保守的功能基因hsp90可以作为研究生物系统发育的手段之一。
英文摘要：
Bemisia tabaci （Gennadius） biotype B and Trialeurodes vaporariorum （Westwood） are worldwide pests. A pair of primers was designed based on the reported sequences of conservative regions of heat shock protein 90 gene （hsp90） family in other insects, and the fragments isolated from the two whitefly species were amplified by RT-PCR, and their full-length cDNA sequences were obtained by 3＇ and 5＇ RACE. The full-length cDNA of hsp90 of two whitefly species contained 2 166 and 2 160 bp open reading frame （ORF） encoding 722 and 720 amino acids, respectively. The deduced amino acid sequence of hsp90 of T. vaporariorum was aligned with that of B. tabaci and both displayed a high degree of conservation （92.94%）. The five highly conserved amino acid residues that characterize all the members of the HSP90 family and the C-terminal pentapeptide MEEVD were found in the two sequences. A phylogenetic tree was constructed based on all the complete amino acid sequences of HSP90 from Insecta by NJ method with genetic distance of Kimura 2-parameter model, which showed that the hsp90 presented good phylogenetic informativeness at both low and high taxonomic levels in Insecta. The results provide new insights into adaptation mechanisms of T. vaporariorurn and B. tabaci to extreme environmental stress. Our data provide further support that the hsp90 sequences with ubiquity and high degree of conservation can provide a useful model for phylogenetic analysis.
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