中文摘要：

以杜仲嫩叶为外植体，诱导愈伤组织并进行增殖试验。在培养基中添加不同浓度的Cu2＋研究Cu2＋对杜仲愈伤组织诱导及增殖的影响。结果表明：诱导培养基中附加1．0mg／L的Cu2＋时，有利于杜仲愈伤组织形成，诱导率最高。在增殖培养基中添加0．7mg／L的Cu2＋时，可明显改善愈伤组织的生长状态，增加愈伤组织增殖生长量。杜仲愈伤组织诱导率及增殖生长量随着Cu2＋浓度的升高而逐渐升高，但当Cu2＋浓度超过1．0mg／L时，诱导率及生长量下降。

英文摘要：

The leaves of E. ulmoides Olive were taken as explants and induced callus formation and proliferation. On the medium,the different concentrations of Cu2＋ were supplemented into the media to study the effects on induction and proliferation of E. ulmoides Olive callus. The results showed that Cu2＋ was advantageous to callus information , and the induction rates were the highest on the induction medium contained 1.0 mg/L Cu2＋. Add 0.7 mg/L Cu2＋ in value-added medium could proliferation growth quantity. The rates of induction and proliferation increased gradually along with the increasing of the concentrations of Cu2＋. But the critical Cu2＋ eontent was 1.0 mg/L.