目的:采用石蜡切片技术制作早期胚胎切片模型。方法:根据阴道脱落细胞检测法确定动情期最佳受孕时间,通过体内受精方法获得小鼠早期胚胎,采用石蜡切片技术制作早期胚胎切片模型。结果:将受孕小鼠断颈处死取各期胚胎,制作含有早胚石蜡切片。结论:本实验组为进一步对早期胚胎发育的研究提供了技术基础。
Objective: To adopt paraffin section technique to obtain the section model of early embryo. Methods: The most favorable fertilization time was confirmed in estrus period by testing vaginal ex foliated cells; the early mice embryo was obtained by internal fertilization, and the early embryonic section model was obtained through paraffin section technique. Results: The pregnant mice was broken the neck to death, embryos of various periods were taken, and the paraffin section containing the early embryos could be made. Results: The experimental team have provided technology basis for further research of the early embryonic development.