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中文摘要：
为了制备培氟沙星（PFLX）抗体,采用N-羟基琥珀酰亚胺活性酯（NHS）法将培氟沙星分别与牛血清白蛋白（BSA）、卵清白蛋白（OVA）偶联,制备免疫抗原PFLX-BSA和检测抗原PFLX-OVA,用PFLX-BSA免疫小鼠以获得高效价的抗体。结果显示,免疫抗原PFLX-BSA紫外光谱具有药物和蛋白的叠加特性,其最大吸收峰在277.5nm处,说明载体蛋白BSA与PFLX成功偶联,可用于动物免疫。检测抗原PFLX-OVA最大吸收峰在275nm处,说明载体蛋白OVA与PFLX成功偶联,可用作检测抗原进行包被。间接ELISA检测结果表明,所得小鼠抗血清效价较高,均达1∶32 000以上,说明有特异性抗体产生。该研究成功制备了抗培氟沙星抗体,也进一步证明药物偶联成功。
英文摘要：
To produce multiclone antibody of PFLX.PFLX were coupled with bovine serum albumin（BSA） and ovalbumin（OVA） by using N-hydroxysuccinimide actived ester method to prepare the immune antigen PFLX-BSA and detection antigen PFLX-OVA.The mice were immunized with PFLX-BSA to obtain the polyclonal antibody with high titer.The ultraviolet spectrum of immune antigen PFLX-BSA had the stacking characteristics of medicament and protein and its maximum absorption peak was 277.5 nm,which indicated that vector protein BSA was successfully coupled with PFLX and it could be used in animal immunization.The maximun absorption peak of detection antigen PFLX-OVA was 275nm,indicating vector protein OVA was successfully coupled with PFLX and it could taken as detection antigen for coating.The results of indirect ELISA detection showed that the antiserum titers of mice were higher and reached over 1∶32 000,which indicated that the specific antibody was produced.The polyclonal antibody against PFLX was successfully prepared,which further proved that PFLX were successfully coupled.
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