中文摘要：

目的：观察隔药饼灸对功能性消化不良（FD）肝郁脾虚模型大鼠HPA轴中枢相关受体及激素的影响,探讨隔药饼灸治疗FD可能的作用机制。方法：将SD大鼠随机分为空白（A）组、模型（B）组、隔药饼灸（C）组、艾炷灸（D）组和逍遥散（E）组,每组10只,除A组外,其余4组采用复合病因造模法造模,连续3周;再按被试因素施加方法,分别进行隔药饼灸、艾炷灸和逍遥散灌胃治疗14d;实验结束后采用RT-PCR检测下丘脑、垂体糖皮质激素受体（GR）m RNA表达,ELISA检测下丘脑促肾上腺皮质激素释放激素（CRH）、垂体促肾上腺皮质激素（ACTH）及肾上腺皮质酮（CORT）的含量。结果：与A组比较,B组大鼠GR m RNA在下丘脑及垂体的表达显著下降（P〈0.01）,且下丘脑CRH、垂体ACTH、肾上腺CORT含量显著上升（P〈0.01）;与B组比较,C、D、E组治疗组大鼠GR m RNA在下丘脑及垂体的表达显著上升（P〈0.01）,且下丘脑CRH、垂体ACTH、肾上腺CORT含量均显著下降（P〈0.01）;与E组比较,C组大鼠GR m RNA在下丘脑及垂体的表达及下丘脑CRH、垂体ACTH、肾上腺CORT含量相当;而D组大鼠GR m RNA在下丘脑及垂体的表达较C、E组较低（P〈0.01）,且下丘脑CRH、垂体ACTH、肾上腺CORT含量较C、E组较高（P〈0.05）。结论：隔药饼灸能显著上调FD模型大鼠下丘脑、垂体GR m RNA表达,下调其下丘脑CRH、垂体ACTH、肾上腺CORT含量,其作用与逍遥散相当,优于艾炷灸。通过纠正HPA轴中枢相关受体及激素的异常表达,抑制HPA轴功能亢进,改善胃肠动力,这可能是隔药饼灸法治疗FD的另一作用机制。

英文摘要：

Objective：To explore the effects of herbal cake-separated moxibustion on related receptors and hormones of HPA axis in rat model of functional dyspepsia（FD） with liver stagnation and spleen deficiency syndrome.Methods：SD rats were randomly divided into five groups：the control group（A）,the model group（B）,the herbal cake-separated moxibustion group（C）,the moxa-cone moxibustion group（D） and the Xiaoyaosan group（E）,10 rats in each group.Except for group A,the rat model of FD with liver stagnation and spleen deficiency syndrome was established by multiple etiological factors including chronic restraint stress,excessive fatigue,irregular food,tail clipping,shaking for 21 consecutive days.The administration of herbal cake-separated moxibustion,moxa cone moxibustion and Xiaoyaosan were separately given to group C,D and E for 14 days.The mRNA expression of GR in hypothalamus and pituitary were detected by RT-PCR,and the levels of corticotropin releasing hormone（CRH） in hypothalamus,adrenocorticotropic hormone（ACTH） in pituitary and corticosterone（CORT） in adrenal were measured by ELISA.Results：Compared with group A,the mRNA expression of GR in hypothalamus and pituitary in group B in duced（P〈0.01）,while the levels of CRH,ACTH,CORT enhanced（P0.01）;Compared with group B,the group C,D,E could enhanced the mRNA expression of GR in hypothalams and pifuitary（P0.01）,while could decreased the levels of CRH ACTH,CORT（P0.01）;The expression of GR mRNA in hypothalamus and pituitary and levels of CRH,ACTH,CORT between group E and group C were similar.The GR m RNA of hypothalamus and pituitary in group D was lower than group C and group E（P0.01）,the levels of CRH,ACTH,CORT in group D were higher than group C and group E（P0.05）.Conclusion：Herbal cake-separated moxibustion could significantly improve the expression of GRmRNA in hypothalamus and pituitary,and decrease the contents of CRH in hypothalamus,ACTH in pituitary,CORT in adrenal,the effects of which are simila