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劲直黄芪水浸提液化感作用研究

ISSN号：1004-5759
期刊名称：《草业学报》
时间：0
分类：S567.01[农业科学—中草药栽培;农业科学—作物学] Q945.7[生物学—植物学]
作者机构：[1]西北农林科技大学动物科技学院,陕西杨凌712100
相关基金：农业部公益性行业专项（200903060）和现代农业产、世技术体系建设专项（CARS-35-02A）资助.

关键词：劲直黄芪, 水浸提液, 化感效应, 抗氧化物酶, Astragalus strictus , aqueous strictus shows a great allelopathic potential. extracts, allelopathic effects, antioxidant enzyme

中文摘要：

采用培养皿滤纸法，首次研究了分布于西藏自治区的劲直黄芪地上部分水浸液对4种受体植物燕麦、反枝苋、白三叶和荠菜种子萌发、幼苗根长、苗高和鲜重的化感效应以及对燕麦和白三叶丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）活性的影响，旨在提示劲直黄芪的化感潜力，为劲直黄芪资源的进一步开发利用提供理论依据。结果表明，劲直黄芪水浸提液对燕麦种子萌发和幼苗生长均表现为抑制作用，且随着劲直黄芪水浸提液浓度的升高其抑制程度不断增强；对反枝苋、白三叶和荠菜种子萌发均表现为抑制作用，幼苗生长基本呈“低促高抑”的化感作用，且浓度达到100．0mg／mL时，反枝苋种子不能萌发，被完全抑制了。不同浓度的劲直黄芪水浸提液影响燕麦和白三叶幼苗体内SOD、POD活性和MDA含量，当浓度为50．0mg／mL时，SOD、POD活性和MDA含量明显增加；浓度升高到100．0mg／mL时，MDA含量继续增加，而SOD和POD活性呈下降趋势。

英文摘要：

Aboveground parts of Astragalus strictus distributed in the Tibet autonomous region, was used to prepare aqueous extracts to test the allelopathic effects on Arena sativa, Amaranthus retroflexus, Triflium repens and Capsella bursa-pastoris using Petridishs with filter papers. Allelopathic effects of aqueous extract were tested on seed germination, root length, seedling height and fresh weight of four species of Astragalus The malondialdehyde （MDA） content, and the activities of superoxide dismutase （SOD）, and peroxidase （POD） of A. sativa and T. repens were investigated to reveal the allelopathic potential of A. strictus, and thereby to provide a theoretical basis for further development and use of A. strictus resources. The aqueous ex tract inhibited seed germination and seedling growth of A. sativa, and the inhibition was enhanced with an in- crease in extract concentration. Seed germination of A. retroflexus, T. repens and C. bursa-pastoris were also inhibited by the aqueous extract, and the effects on seedl effect＇ , promoting growth at the lower concentrations and in mg hib growth presented an obvious ＇concentration iting growth at the higher ones. A.retroflexus seed germination was inhibited completely when the aqueous extract concentration was 100 mg/mL. The aetivi ties of SOD, POD and MDA contents of A. sativa and T. repens were affected by different concentrations of a queous extract. SOD, POD activities and MDA content markedly increased at 50 mg/mL aqueous extract, whereas at 100 mg/mL, the extract increased MDA content and reduced the enzyme activity in the seedlings of A. sativa and T. repens. Therefore, A.

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