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中文摘要：
设计合成了一种用于检测Hg2＋的苯并噁唑类荧光探针4-（苯并[d]噁唑-2-基）-2-（（二（2-（乙巯基）乙基）氨基）甲基）苯酚（ZY1）,用质谱、核磁氢谱和X射线单晶衍射等方法对其进行了结构表征,并考察了其光谱性能.研究结果表明,ZY1对Hg2＋有较高的选择性和灵敏度,它们之间的结合比为1∶1,共存离子及pH值（3.0~9.0）对其测定影响小.ZY1能够可视化检测Hg2＋,并在HeLa活细胞中实现了汞离子的成像,这对检测生物体内的Hg2＋将具有潜在的应用价值.
英文摘要：
A benzoxazole-based fluorescent probe 4-（benzo[d]oxazol-2-yl）-2-（（bis（2-（ethylthio）ethyl）amino）methyl）phenol （ZY1） was designed and synthesized, and characterized by mass spectrometry （MS）, nuclear magnetic resonance （N M R） and X-ray single crystal diffraction, etc. ZYI was prepared by Mannich reaction, and its pale yellow crystals suitable for X-ray diffraction studies were obtained by slow evaporation of an ethyl acetate solution at room temperature over a period of a few days. The results showed that ZY1 exhibited good sensitivity and selectivity to Hg2＋. The fluorescence of ZYl was quenched dramatically in the presence of Hg2., and it remained nearly constant in the range of pH 3.0~9.0. Other metal ions, such as K＇, Ca2-, Na＋, Mg〉, Mn2 ~, Fe2~, Fe3＋, Co2~, Ni2＋, Cu2~, Zn2＋, Cd2＋, Pb2＋, induced negligible fluorescence quenching for ZY1 under the same conditions. When the concentration of Hg2＋ was increased from 0 to 30 ~tmol/L, the fluorescence of ZY1 （10 lamol/L） quenched gradually. Binding analysis using the method of continuous variations （Job＇s plot） established that the stoichiometry of ZYI-Hg2L complex was estimated to be l ： l, The fluorescence intensity varied almost linearly vs, the concentration of Hg2＋ （0.5~4.8 gmol/L）, and the detection limit of Hg2 ~ was estimated to be 0.08 gmol/L. Moreover, ZYI was used for the visual detection of Hg2＋ and applied to image intracelluar Hg2＋ in living HeLa cells, indicating that it could be a useful tool in bioanalytical applications.
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