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脊髓背根神经节神经元瞬时受体电位离子通道1在大鼠糖尿病神经痛形成中的作用

ISSN号：0254-1416
期刊名称：《中华麻醉学杂志》
时间：0
分类：R338.3[医药卫生—人体生理学;医药卫生—基础医学]
作者机构：[1]贵州省人民医院麻醉科,贵阳市550002, [2]贵阳医学院研究生院, [3]贵州省人民医院神经内科,贵阳市550002
相关基金：贵阳市科技局项目（[2012103]53）;贵州省青年英才培养项目（黔省专合字[2012]198）;贵州省卫生厅基金（2011gzwkj2011-1-009）

作者：邹晋峰[1], 贺纯静[1], 李锶蕊[2], 余倩[3], 高华琳[1]

关键词：瞬时受体电位通道, 后角细胞, 糖尿病神经病变, Transient receptor potential channels, Posterior horn cells, Diabetic neuropathies

中文摘要：

目的评价脊髓背根神经节神经元瞬时受体电位离子通道1（TRPA1）在大鼠糖尿病神经痛形成中的作用.方法糖尿病神经痛模型制备成功的SD大鼠24只,采用随机数字表法分为3组（n=8）：糖尿病神经痛组（DNP组）、TRPA1 siRNA组（siRNA组）和TRPA1-阴性siRNA组（NC组）.另取血糖正常的SD大鼠8只,作为正常对照组（C组）.siRNA组鞘内注射TRPA1 siRNA 45 μL,NC组鞘内注射TRPA1-阴性siRNA 45 μL,DNP组和C组鞘内注射生理盐水45μL.各组于鞘内注药后第2天,取L4-6背根神经节,采用RT-PCR法测定神经元TRPA1 mRNA的表达,于鞘内注药后第7、14、21和28天（T1-4）时测定机械缩足反应阈（MWT）.结果与DNP组比较,siRNA组和C组脊髓背根神经节神经元TRPA1 mRNA表达下调（P＜0.05）;与siRNA组比较,NC组脊髓背根神经节神经元TRPA1 mRNA表达上调（P＜0.05）;与NC组比较,C组脊髓背根神经节神经元TRPA1 mRNA表达下调（P＜0.05）.与DNP组比较,siRNA组T1.2时、NC组T1-3时MWT降低,C组T1-4时MWT升高（P＜0.05）;与siRNA组比较,C组T1-4时MWT升高（P＜0.05）;与NC比较,C组T1-4时MWT升高（P＜0.05）.结论脊髓背根神经节神经元TRPA1参与大鼠糖尿病神经痛的形成.

英文摘要：

Objective To evaluate the role of transient receptor potential ankyrin 1 （TRPA1） in the dorsal root ganglion neurons in the development of diabetic neuropathic pain （DNP） in rats.Methods Twenty-four Sprague-Dawley rats with DNP were randomly divided into 3 groups （n-=8 each） using a random number table：DNP group,TRPA1-specific siRNA group （siRNA group） and TRPA1-negative siRNA group （NC group）.Another 8 Sprague-Dawley rats with normal blood glucose served as control group （C group）.In siRNA group,TRPA1-specific siRNA 45 μl was injected intrathecally.In NC group,TRPA1-negative siRNA 45 μl was injected intrathecally.In DNP and C groups,normal saline 45 μl was injected intrathecally.On 2nd day after intrathecal administration,the lumbar segment （L4-6） of the dorsal root ganglions was removed for determination of the expression of TRPA1 mRNA.On 7,14,21 and 28 days after intrathecal administration （T1-4）,MWT was measured.Results Compared with DNP group,TRPA1 mRNA expression was down-regulated in siRNA and C groups.Compared with DNP group,and MWT was significantly decreased at T1.2 in siRNA group,MWT was decreased at T1-3 in NC group,MWT was increased at T1-4 in group C.Compared with siRNA group,MWT was significantly increased at T1-4 in group C.MWT was significantly higher at T1~ in group C than in NC group.Conclusion TRPA1 in the dorsal root ganglion neurons is involved in the development of DNP in rats.

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