为探明喀斯特适生植物土人参光合作用日进程特征及其关键影响因子,为土人参的科学栽培提供理论依据,采用Li-6400光合作用测定系统测定土人参叶片光合生理生态特性及环境因子日变化,并进行系统分析。结果表明,一天中,土人参气孔导度(Gs)为3峰曲线;胞间CO2摩尔分数(Ci)大致为"V"字型曲线;蒸腾速率(Tr)为双峰曲线;水分利用效率(WUE)与光能利用效率(LUE)早间最高,总体上逐渐下降。土人参净光合速率(Pn)日进程大致表现为双峰曲线,第1峰(峰值9.82μmol.m-2.s-1)出现在9:00左右,第2峰(峰值9.25μmol.m-2.s-1)出现在16:00左右,光合"午休"现象较为明显。相关分析、回归分析及通径分析表明,土人参Pn与空气相对湿度(RH)、Gs、Tr以及光合有效辐射(PAR)显著正相关,而与Ci显著负相关;光合"午休"的原因主要为气孔关闭引起的气孔限制因素;Gs、Ci及Tr是Pn的关键影响因子,Gs影响最大,Ci与Tr次之。
In order to explore the characteristics of diurnal variation of photosynthesis and its key in- fluencing factor of karst adaptable pant Talinum paniculatum , accordingly providing a theoretical ba- sis for the cultivation of scientific cultivation,the eco-physiological photosynthetic characteristics of T. paniculatum and the environmental factors were measured using Li-6400 portable photosynthesis sys- tem. The results were analyzed systematically and summarized as follows, the diurnal variation of stomatal conductance(Gs) of T. paniculaturn showed a 3 peaks curve~ the diurnal variation pattern of intercellular CO2 concentration(C~) showed a "V"-type curve~ the diurnal variation curves of leaf tran- spiration(Tr) was twin-peaked~ the values of water use efficiency (WUE) and light use efficiency (LUE) were the highest at the morning in a day,and gradually decreased overall. The diurnal change of net photosynthetic rate (Pn) of T. paniculatum presented a bimodal pattern roughly,the first peak (Pn=9.82μmol·m-2·s-1) appeared at 9 o’clock and the second peak(Pn=9.25μmol·m-2 ·s-1) appeared at 16 o‘clock,and in midday there was a obvious photosynthetic depression. The correlation analysis,regression analysis and path analysis showed that the diurnal variations of Pn significantly and positively related to that of relative air humidity (RH), Gs, photosynthetically active radiation (PAR) and Tr ,but significantly and negatively related to that of C~. The main reason of midday photo- synthetic depression was stomatal limitation factors based on stomata closure. Gs, Ci and Ti were the key influencing factors on Pn of T. paniculatum,and Gs produced more influence than Ci and Tr.