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中文摘要：
为在真核细胞中表达牛乳头瘤病毒2型（BPV2）衣壳蛋白L1基因。采用PCR方法从奶牛皮肤肿瘤病料中扩增BPV2沙湾分离株L1基因,克隆入pMD18-T载体中,测序后进行序列分析。以pcDNA 3.1（＋）为真核表达载体构建重组表达质粒,转染入BHK-21细胞,通过间接荧光免疫试验检测BPV2-L1蛋白的表达。本研究成功构建pcDNA3.1（＋）-BPV2-L1重组真核表达质粒,证实能表达出BPV2-L1蛋白,为BPV2DNA疫苗的研发提供了前期基础。
英文摘要：
To express of L1 gene of bovine papillomavirus type 2 （BPV2-L1） in BHK-21 cells. BPV2 L1 gene was amplified from sample of skin lesions by PCR. Then the product was cloned into pMD18- Tvector, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1（＋）. The recombined vector was transfected into BHK-21 cells with lipofectin and the expression of L1 gene was detected by indirect immunofluorescence assay. The eukaryotic expressing vector encoding BPV2 L1 was successfully constructed and its expression in BHK-21 cells could be detected. The eukaryotic expressing vector encoding BPV2 L1 was constructed and it expressed L1 protein correctly in BHK-21 cells.
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