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由沙门氏菌引起的犊牛腹泻病的实验室诊断及防治

ISSN号：1671-7236
期刊名称：《中国畜牧兽医》
时间：0
分类：S858.23[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医]
作者机构：[1]中国农业大学动物医学院,北京100193, [2]北京市动物疫病预防控制中心,北京102600, [3]新疆维吾尔自治区动物卫生监督所,乌鲁木齐830011
相关基金：国家奶牛产业技术体系（CARS-37）; 公益性行业（农业）科研专项经费（201303037）

作者：张树栋[1], 李豪[1], 万强[1], 徐发荣[2], 居马别克·夏拉巴依[3], 潘保良[1]

关键词：犊牛腹泻, 沙门氏菌, 诊治, calf diarrhea, Salmonella, diagnosis and treatment

中文摘要：

为了探究山西某规模化奶牛场犊牛腹泻病的发病原因及相应的综合防治措施,本研究通过对该场犊牛腹泻病发病情况进行调查,同时对可疑病原轮状病毒、冠状病毒、大肠杆菌F5（K99）、小球隐孢子虫、沙门氏菌进行实验室检测,分离并培养了可疑病原菌,并对该病原菌进行形态学和血清学鉴定,选取18种常见抗生素,通过药敏试验筛选对该菌敏感的抗生素。结果显示,经临床诊断和实验室检测基本排除大肠杆菌F5（K99）、轮状病毒、冠状病毒、小球隐孢子虫和球虫感染的可能性,分离的可疑菌具有沙门氏菌的菌落特征和细菌形态,并与沙门氏菌O多价血清A-F呈现阳性反应,确诊该病原主要为沙门氏菌。药敏试验结果显示,所分离的沙门氏菌对头孢曲松、庆大霉素、头孢拉定、阿米卡星等药物高度敏感,可推荐用于临床治疗。结合以上结果,本研究提出该病的具体防治措施,并取得了较好的防治效果。本研究初步为犊牛腹泻病中沙门氏菌病的诊断和防治提供了参考,对犊牛腹泻病病原的早期诊断和治疗具有重要的临床意义。

英文摘要：

This study was aimed to explore the pathogenesis of calf diarrhea in a large scale dairy farm in Shanxi province and the correspond comprehensive prevention and control measures.By investigating the incidence of calf diarrhea in the field,rotavirus,coronavirus,E.coli F5（K99）,Cryptosporidium parvum,coccidia and Salmonella were detected through experimental diagnosis,and the suspicious pathogenic bacteria was isolated and cultivated,which was identified with morphology and serology,simultaneously sensitive antibiotics were screened according to the antibiotic susceptibility tests,which contained 18 kinds of common antibiotics.The results showed that the clinical diagnosis and laboratory tests basically ruled out the possibility of E.coli F5（K99）,rotavirus,coronavirus,Cryptosporidium parvumand coccidia infection,and the isolated suspected bacteria had Salmonellacolony characteristics and bacterial morphology,which showed positive reaction to Salmonella O polyvalent A-F serum,so we had identified that it was Salmonella diarrhea.The antibiotic susceptibility tests results showed that Salmonella was highly sensitive to ceftriaxone,gentamicin,cephradine and amikacin,which could be recommended for clini-cal treatment.Above all,this study proposed the specific prevention and treatment of the disease,and better control effect was achieved.This study initially provided a reference for the diagnosis and treatment of Salmonellaof calf diarrhea,with great clinical significance in the early diagnosis and treatment of pathogens of calf diarrhea.

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