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中文摘要：
病原菌的相互作用组学研究是揭示潜在信号转导通路和新型药物靶标的有力工具。通过同源蛋白映射的方法构建了牛支原体的蛋白-蛋白相互作用网络,所构建的互作网络包含138个蛋白和693个相互作用关系。通过蛋白的互作频率分析,确定了在牛支原体中互作频率最高的20个蛋白,这些蛋白主要与转录、翻译、分子伴侣等功能相关。进一步分析发现,伴侣蛋白DnaK和ClpB发生互作的频率较高,能与核糖体蛋白、代谢相关蛋白等多种功能蛋白发生相互作用。结果提示,若DnaK和ClpB的功能受到抑制,将会对牛支原体的正常生命活动造成较大的影响。
英文摘要：
Analysis of the pathogen interactome is a powerfull approach for dissecting potential signal trasducion and new drug targets.We constructed a comprehensive protein-protein interaction network for My coplasmabovisconsisting of 138 proteins and 693 interaction pairs.Our analysis unraveled 20 proteins with highest interactions in the network,most of them are related to the function of transcription,translation and molecular chaperone.The MMB0149/MMB0664 gene and its gene product DnaK/ClpB were analyzed in detail because they showed high interactions with ribosomal and metabolism related proteins.Our analyses indicated that the chaperone DnaK and ClpB may be critical for the survival of Mycoplasma bovis.Collectively,this study therefore provides valuable clues in exploring new signaling protein and new drug targets.
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