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中文摘要：
建立一种QuEChERS-超高效液相色谱-串联质谱（QuEChERS-UPLC-MS/MS）检测畜禽组织中金刚烷胺残留的方法。样品用1%乙酸乙腈溶液超声提取，提取液加入C18和PSA填料进行基质分散固相净化，浓缩后经Proshell 120 EC-C18柱分离，用电喷雾离子源正离子多反应监测（MRM）模式进行检测。在0.2μg/L～100.0μg/L线性范围内具有较好的线性关系，相关系数大于0.997。该方法检出限（LOD）为0.6μg/kg，定量限（LOQ）为2.0μg/kg，回收率为70.5%～100.1%，相对标准偏差（RSD）小于10%。该方法简便快速、准确度好，适用于畜禽组织中金刚烷胺药物残留的检测。
英文摘要：
A method for the determination of the residues of amantadine in livestock and poultry tissues by QuEChERS extraction and ultra high performance liquid chromatograph-tandem mass spectrometric method （UPLC-MS/MS） was established. The sample was extracted with acetonitrile （containing 1%acetic acid） and followed by ultrasound. Cleanup of the extracts was processed by C18 and PSA , a modified dispersive solid phase extraction （QuEChERS）. The analysts were then separated by Poroshell 120 EC-C18 column, and finally detected by tandem mass spectrometry in positive ESI mode. The linearity of amantadine in the range from 0.2 μg/kg to 100.0μg/kg had correlation coefficient greater than 0.997. The limits of detection （LOD） of the method were 0.6μg/kg and the limits of quantification （LOQ） were 2.0μg/kg. The average recoveries of amantadine were in the range from 70.5%to 100.1%, with relative standard deviations were less than 10%.The established method is sensitive and of good recoveries. It applies to the determination of the residues of amantadine in poultry tissues and meat.
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