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硒对碘过量仔鼠脑5＇-和5-脱碘酶活性的干预作用

ISSN号：1001-1889
期刊名称：《中国地方病防治杂志》
时间：0
分类：O613.52[理学—无机化学;理学—化学] O613.44[理学—无机化学;理学—化学]
作者机构：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030, [2]湖北省郧阳医学院预防医学教研室
相关基金：国家自然科学基金重点项目（30230330）和湖北省教育厅基金（2002A03007）资助

作者：郭怀兰[1,2], 杨雪锋[1], 徐健[1], 侯晓晖[1], 陈骁熠[1], 孙秀发[1]

关键词：高碘, 硒, 脱碘酶, 仔鼠, 大脑, Iodine excess intake, Selenium, Iodothyronine deiodinase, Filial mice, Cerebrum

中文摘要：

目的研究小鼠摄入高碘对仔鼠脑5＇-和5-脱碘酶活性的影响及硒的干预作用。方法将60只Balb／c断乳小鼠按体重随机分为4个不同的饮水组：正常对照组、高碘组（3000μg／L I）、单独补硒组（200μg／L Se）、高碘加硒组（3000μg／LI＋200μg／L Se），喂养4个月时，雌雄交配。测定14和28日龄仔鼠血清TSH、TT4、TT3和rT3水平，及0、14和28日龄仔鼠大脑5＇-和5-脱碘酶活性。结果14日龄仔鼠血清TT4水平高碘组显著低于对照组和高碘加硒组，而TSH水平高碘组显著高于对照组和高碘加硒组，28日龄仔鼠血清TT4、TT3和TSH水平各组间差异无统计学意义（P〉0．05）。0和14日龄仔鼠脑5＇-脱碘酶活性高碘组显著高于对照组（P〈0．05），高碘补硒组显著低于高碘组。0和14日龄仔鼠脑5-脱碘酶活性高碘组显著低于对照组（P〈0．05），而高碘加硒组显著高于高碘组。结论补硒能缓解高碘所致仔鼠脑5＇-和5-脱碘酶活性的改变。

英文摘要：

Objective To study the influence of iodine excess intake on the activity of iodothyronine 5＇- and 5 - deiodinase in the cerebrum of filial mice and the effect of selenium intervention. Methods Sixty Balb/c mice were divided randomly into four groups with different drinking water. They were control group, iodine excess group （3000 μg/L I）, selenium supplementation group （200 μg/L Se）, and iodine excess plus selenium group （3000 μg/L I and 200 μg/L Se）. At the end of the fourth month, the mice were mated. Serum TT4, TT3, rT3 and TSH of the progenies were determined on postnatal day 14 and 28. Activities of T4 5＇- deiodinase and 5 - deiodinase in the cerebrum of filial mice were measured too. Results On postnatal day 14, serum TT4 level was lower, and serum TSH was significantly higher in the iodine excess group than the control group and higher than the iodine excess plus selenium group. On postnatal day 28, no significance in serum TT4, TT3 and TSH level among the four groups. On postnatal day 0 and 14, iodothyronine 5＇- deiodinase activities in the cerebrum of the progenies in the iodine excess group were significantly higher than those in the control group , while the iodine excess plus selenium group were lower than those in the iodine excess group （ P 〈 0.05） ; iodothyronine 5 - deiodinase activity in the excess iodine group was significantly lower than those in the control group （ P 〈0.05）, while the iodine excess plus selenium group was higher than those in the iodine excess group （ P 〈 0.05）. Conclusions Iodine excess intake can change the activities of iodothyronine 5＇- and 5 - deiodinase in the cerebrum of filial mice and selenium supplementation might have some favorable effects.

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