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碘过量对仔鼠脑突触素表达影响及硒干预作用

ISSN号：1001-0580
期刊名称：《中国公共卫生》
时间：0
分类：R151.2[医药卫生—营养与食品卫生学;医药卫生—公共卫生与预防医学] R599[医药卫生—临床医学;医药卫生—内科学]
作者机构：[1]郧阳医学院太和医院,湖北十堰442000, [2]郧阳医学院预防医学教研室, [3]华中科技大学同济医学院公共卫生学院营养与食品卫生学系
相关基金：国家自然科学基金重点项目（30230330）; 湖北省卫生厅项目（JX3B30）

作者：郭怀兰[1,2], 张吉才[1], 王建洲[2], 王静[2], 杨雪锋[3], 孙秀发[3]

关键词：碘过量, 硒, 突触素, 大脑, excess iodine, selenium, synaptophysin, cerebrum

中文摘要：

目的研究碘过量摄入对小鼠仔鼠脑突触素表达影响及硒的干预作用。方法将60只BALB/c小鼠随机分为4组：正常对照组（饮用自来水）、碘（I）过量组（饮水3000μg/LI）、单独补硒（Se）组（饮水200μg/LSe）、碘过量加硒（饮水3000μg/LI＋200μg/LSe）组。饲养4个月后,雌雄交配。测定第14和28d仔鼠血清总四碘甲状腺原氨酸（T4）和总三碘甲状腺原氨酸（T3）水平,分别用荧光定量聚合酶链式反应（PCR）和蛋白印迹试验（Western blot）测定仔鼠大脑组织突触素mRNA和蛋白的表达。结果第14d碘过量组仔鼠血清T4水平为（68.78±11.10）nmol/L,明显低于正常对照组和碘过量加硒组的（100.85±11.47）和（93.15±12.10）nmol/L（P〈0.05）; 其脑组织突触素mRNA相对量为（44.87±3.82）,明显低于正常对照组、单独补硒组和碘过量加硒组的（58.34±8.13）,（54.33±5.32）,（49.89±7.46）; 其蛋白相对量为（0.64±0.02）,明显低于正常对照组、单独补硒组和碘过量加硒组的（0.78±0.01）,（0.76±0.01）,（0.81±0.03）（P〈0.05）。碘过量和补硒对第28d仔鼠血清T4和T3水平和脑组织突触素表达水平无明显影响。结论碘过量引起仔鼠脑突触素表达异常,补硒有缓解作用。

英文摘要：

Objective To study effects of excess iodine intake on synaptophysin expression in the cerebrum of filial mice and the intervention of selenium.Methods Sixty BALB/c mice were divided randomly into four groups with different drinking water：control group （tap water）,excess iodine group （3 000 μg/L）,supplementing selenium group（200 μg/L） and excess iodine plus selenium （3000 μg/L of iodine＋ I 200 μg/L of selenium） group.The mice were mated at the end of the fourth month.Serum T4 and T3 were determined on 14th and 28th postnatal day.The expression level of synaptophysin in filial cerebrum was measured with fluorescent real time PCR and Western blot.Results Serum T4 level in excess iodine group（68.78±11.10 nmol/ L） was lower significantly than that of in control group （100.85±11.47 nmol/ L） and excess iodine plus selenium group （93.15±12.10 nmol/ L） on 14th postnatal day.mRNA and protein level of synaptophysin in excess iodine group（relative mRNA and protein level was 44.87±3.82 and 0.64±0.02） was lower than that in other groups （relative mRNA level was 58.34±8.13,54.33±5.32,49.89±7.46,relative protein level was 0.78±0.01,0.76±0.01,0.81±0.03,respectively） on 14th postnatal day（P0.05）.No significant difference was observed in serum T4 and level of synaptophysin between four groups on 28th postnatal day.Conclusion Excess iodine intake can change the expression of synaptophysin in cerebrum and selenium of filial mice and selenium supplementation can alleviate it.

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