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中文摘要：
目的观察碘过量对自身免疫性疾病敏感品系NOD鼠和非敏感品系Balb／c鼠甲状腺细胞凋亡相关基因肿瘤坏死因子的相关凋亡诱导配体（tumor neerosis faetor—related apoptosis—indueing ligand，TRAIL）和其受体TRAIL刺激性受体1（TRAIL—sR1）表达的影响，了解碘过量诱导实验性自身免疫性甲状腺炎（EAT）发病过程中TRAIL和TRAIL-sR1的作用机制。方法6～7周龄雌性NOD鼠和Balb／c鼠各16只，按体质量各随机分为对照组和碘过量（HI）组，每组8只。对照组饮高压灭菌水，HT组饮0．05％NaI添加水，饲养8周后处死。测量甲状腺相对质量，进行甲状腺组织形态学观察，测量血清甲状腺素（仉）和甲状腺刺激激素（TSH），检测甲状腺滤泡上皮细胞凋亡情况，应用实时定量PCR（realtimePCR）方法检测TRAIL和TRAIL—sR1mRNA表达情况。结果NOD鼠HI组甲状腺相对质量[（104．8±14．5）mg／kg]高于对照组[（71．8±20．4）mg／kg]，血清矾水平[（30．77±3．59）mmol／L]低于对照组[（36．43±2．66）mmol／L]，TSH水平[（6．98±0．66）μg／L]高于对照组[（5．55±0．56）μg／L]，组间比较差异均有统计学意义（f值分别为7．773、-9．526、-4．458，P均〈0．05）。HI组甲状腺出现滤泡扩张、胶质潴留及明显的淋巴细胞浸润伴灶性纤维化。Balb／c鼠HI组甲状腺相对质量[（155．8±20．8）mg／kg]高于对照组[（105．1±22．0）mg／kg]，血清TT4水平[（19．75±3．32）mmol／L]低于对照组[（23．46±6．21）mmol／L]，TSH水平[（4．14±1．71）μg／L]高于对照组[（3．55±1．41）μg／L]，组间比较差异均有统计学意义（￡值分别为7．554、-7．239、3．140，P均〈0．05）；HI组甲状腺仅有甲状腺滤泡扩张，胶质潴留，而未见明显的淋巴细胞浸润。HI组NOD鼠和Balb／c鼠甲状腺滤泡上皮细胞的凋亡指数（3．97±0．91、1．05±0．45）分别高于对照组（0．21?
英文摘要：
Obiective To investigate the influence of iodine excess on expression of TRAIi/TRAIL-sR1 in NOD and Balb/c mice and to study the effect of TRAII/TRAIL-sR1 on the pathogenesis of experimental autoimmune thyroiditis（EAT）. Methods Both Balb/c and NOD mice were divided randomly into control and iodine excess group by feeding with water containing no NaI or 0.05% NaI. The mice were sacrificed after 8 weeks. TRAIL and TRAIL-sR1 mRNA levels were detected by RT-PCR. The function, morphology and apoptosis of thyroids were also observed by ELISA and Tunnel stain. Results Treated by HI, enlarged follicles and flattened epithelium by accumulation of colloid were found in thyroids of both NOD and Balb/c mice. But significant lymphoid cell infiltration and local fibrosis were only found in thyroids of NOD HI group. The relative weight of thyroids of NOD mice in HI group [ （ 104.8 ± 14.5 ） mg/kg ] was heavier than that of control group [ （71.8 ± 20.4 ）mg/kg ]. The level of TT4 declined in HI group[ （30.77± 3.59）mmol/L] compared with control group[ （36.43 ± 2.66）mmol/L], meanwhile, the level of TSH was higher in HI group[ （6.98 ± 0.66）μg/L] than that in control group[（5.55 ± 0.56）μg/L]. The difference being statistically significant（t = 7.773± 9.526,- 4.458, all P 〈 0.05）. The relative weight of thyroids of Balb/c mice of HI group [ （155.8 ± 20.8）mg/kg] also heavier than that of control group [ （105.1 ± 22.0）mg/kg]. The level of TT4 droped in HI group[ （19.75 ± 3.32）mmol/L] was higher than that in control group [ （23.46 ± 6.21 ）mmol/L], the level of TSH in HI group [ （4.14 ± 1.71 ）μg/L] was higher than that in control group [ （3.55 ±1.41 ）μg/L], the difference being statistically significant （t = 7.554, - 7.239,3.140, all P 〈 0.05）. A great deal of apoptotic cells observed in NOD （3.97 ± 0.91 ） and Balb/c mice （1.05 ± 0.45 ） by Tunnel stain were greater than control groups （0.21 ± 0.15, 0.10 ± 0.03）, the difference being statisticall
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