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中文摘要：
目的通过研究观察过量碘对甲状腺细胞凋亡相关基因的影响,探讨过量碘促进甲状腺细胞凋亡的机制。方法选取FRTL细胞系为研究对象,正常对照组培养基内不加碘,过量碘组分别含碘（碘化钾）1、5、10、50和100mmol/L,培养24h,流式细胞术测定凋亡细胞的比例,荧光定量PCR法测定5、10、50mmol/L碘剂量组fas、fasL、Bcl-2和Bax的 mRNA水平。结果与正常对照组比较,10、50和100mmol/L碘剂量组FRTL细胞凋亡率显著升高（P〈0.05）。5mmol/L碘组各项指标与正常对照组比较差异无显著性。10mmol/L和50mmol/L碘组fasL和Bax mRNA水平较正常对照组显著升高（P〈0.05）,Bcl-2 mRNA水平较正常对照组显著下降（P〈0.05）。10mmol/L碘组fas与正常对照组比较差异无显著性,50mmol/L碘组fas较正常对照组显著升高（P〈0.05）。结论过量碘可通过调节fas、fasL、Bcl-2及Bax的表达而促进影响甲状腺细胞凋亡的发生。
英文摘要：
Objective To explore the mechanism of iodine over-treatment to FRTL cell on the cell apoptosis through observing the cell apoptosis related gene. Methods The cells were treated with potassium iodide （0, 5, 10, 50 and 100mmol/L）. After treatment 24 hours, the cells were collected and analyzed by flow cytometry. The mRNA levels of fas, fasL, Bcl-2 and Bax of 50mmol/L and 100mmol/L groups were determined with real-time PCR. Results Compared with the normal control group, the rates of cell apoptosis of 10, 50 and 100mmol/L iodine groups increased significantly. Compared with the normal control group, the mRNA levels of fasL and Bax in 10 and 50mmol/L iodine groups increased significantly while the level of Bcl-2 decreased significantly. There was no significant difference fas mRNA levels between the normal control group and 10mmol/L iodine group, but the fas mRNA level in 50mmol/L iodine group increased significantly. Conclusion The excessive iodine treatment increased the apoptosis of FRTL cells which might be related to gene expressions of fas, fasL, Bcl-2 and Bax.
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