通过N^+注入解脂耶罗威亚酵母(Yarrowia lipolytica)DC-3-2,筛选出一株油脂降解率提高了11.09%的高效菌株DC.3.2.50,经10次传代实验表明该菌株遗传稳定向良好。对DC-3-2-50的降解条件进行了初步研究,结果表明,在最佳条件:初始pH9.0、接种量3.0%、温度为25--28℃、摇床转速为180-200rpm、碳源为大豆油时,其降解率最高,可达87.7%。
A strain DC-3-2-50 was obtained through N^+ implanting into Yarrowia lipolytica DC-3-2. An increase of 11.09% in the oil-degradation rate was obtained. The stain has good genetic stability after 10 times of subculture. The culturing condition of DC-3-2-50 was studied. The optimal culture conditions were as follow: initial pH value, 9.0; inoculum size, 3%; temperature, 25--28℃; dissolved oxygen, 180-200 rpm; and carbon nutriments soybean salad oil. The oH-degradation rate can be up to 87.7%.